[Histonet] Cyclin D1 antibody from Cell Marque user experiences

Dana Settembre settembr <@t> umdnj.edu
Mon Jun 11 12:38:21 CDT 2007


Sarah,  I use the same Lab Vision Cyclin D1, rabbit monoclonal as
mentioned below.


Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA


>>> "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com> 06/11/07 11:23 AM
>>>
Sarah,

I use Lab Vision's bcl-1(Cyclin D1) and it looks great!  It is an ASR
and is a rabbit monoclonal.

Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Sarah
O'Bryan
Sent: Monday, June 11, 2007 1:15 AM
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] Cyclin D1 antibody from Cell Marque user
experiences


 

Hello all,

 

I would like some information or feedback on how people find different
Cyclin D1 antibodies? I can see from some previous messages that it
can
be a difficult antibody to optimize... I am particularly interested in
Cell Marque's users, as we have a customer who would like to know of
user experiences with this antibody before she decides whether to
trial
or not.

 

If you use Cyclin D1 in your laboratory, would you please let me know
what clone you use, who manufactures it, and what you think of it?
What
is the leading antibody on the market?

 

Thanks for your help.

 

Sarah

 

 

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