[Histonet] Laser capture microscopy to isolate sebocytes

[Sherwood, Margaret]

Luminita,

I referred you to Molecular Devices (Arcturus Division).  I'm not sure what LCM
instrument you are using, but they sell the kits you are interested in.  

Peggy

Peggy Sherwood 
Lab Associate, Photopathology 
Wellman Center for Photomedicine (W224) 
Massachusetts General Hospital 
55 Fruit Street 
Boston, MA 02114-2696 
617-724-4839 (voice mail) 
617-726-6983 (lab) 
617-726-1206 (fax) 
msherwood <@t> partners.org 



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
Castillos, Luminita
Sent: Wednesday, June 06, 2007 10:42 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Laser capture microscopy to isolate sebocytes


Hi everybody, 

 

Does somebody have experience on laser capture microscopy to isolate
human sebocytes? I have some skin punch biopsies and I need to perform
LCM to isolate sebocytes for RNA purification, amplification and gene
expression analysis. I am worried about: what type of staining I should
perform? What kind of RNA extraction kit I should use for these
particular cells and what is the RNA yield I should expect? I deeply
appreciate any feedback for this particular issue. Thank you in advance.

 

L, 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information.