[Histonet] Perfusing Rat Lung

[Alexina Wempren]

Does anyone have a good protocol for perfusing rat lung?  We have been
flushing with ringers, then 10% NBF, then ringers and ending with 30%
sucrose overnight but the morphology is not good. We add 0.8cc of air so
the airways and vessels are not flat but it's still not great. We used
to use paraformaldehyde but my supervisor did not like it. Any help
would be greatly appreciated.

   Thanks,           alexina