[Histonet] DNA extraction method from archival Giemsa stained bonemarrow smears

Sherwood, Margaret MSHERWOOD <@t> PARTNERS.ORG
Fri Jun 1 13:02:28 CDT 2007


Luminita,

I refer you to Molecular Devices-Arcturus Division.  They sell the PicoPure DNA
extraction kit--it is used in conjunction with the Paradise Reagent Kit for FFPE
tissue.  You can either use it for laser capture microdissection (LCM) or tissue
you have scraped off the slide.  

Peggy

Peggy Sherwood 
Lab Associate, Photopathology 
Wellman Center for Photomedicine (W224) 
Massachusetts General Hospital 
55 Fruit Street 
Boston, MA 02114-2696 
617-724-4839 (voice mail) 
617-726-6983 (lab) 
617-726-1206 (fax) 
msherwood <@t> partners.org 



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
Castillos, Luminita
Sent: Friday, June 01, 2007 1:17 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] DNA extraction method from archival Giemsa stained
bonemarrow smears


Hi everyone, 

 

Does anyone have any experience with using a good method to extract DNA
from archival Giemsa stained bone marrow smears?. Is there available a
commercial kit for the DNA extraction?. Thank you in advance and have a
great Friday.

 

Sincerely, Luminita

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