[Histonet] Fat issues

[Cheri Miller]

I meant to add that the x-tra time in 100% and xylene melts the fat away. 

Cheri Miller HT ASCP
Histology Supervisor
Physicians Laboratory Services, Inc.
Omaha, NE 68117
402 738 5052

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-----Original Message-----
From: Cheri Miller [mailto:cmiller <@t> physlab.com] 
Sent: Monday, July 30, 2007 8:00 AM
To: 'mickie25 <@t> netzero.net'; 'Joe Nocito'; 'AGrobe2555 <@t> aol.com';
'histonet <@t> lists.utsouthwestern.edu'
Subject: RE: [Histonet] Fat issues

I melt down the block, recap and run it thru my clean cycle on the VIP and
then place back in formalin and run with the next days cases. It works
beautifully. Cheri

Cheri Miller HT ASCP
Histology Supervisor
Physicians Laboratory Services, Inc.
Omaha, NE 68117
402 738 5052

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mickie
Johnson
Sent: Friday, July 27, 2007 8:12 PM
To: 'Joe Nocito'; AGrobe2555 <@t> aol.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Fat issues

Hello Albert, Joe and all you dedicated Histonetters! Happy Friday!

I wrote an article in the Histo-Logic about 4 years ago,  (It is in the
archives) about a very useful technique for coping with this problem. Here
it is in a nut shell.

1. Melt the block down. Save the cassette
2. Blot off all wax. Don't press as this distorts the tissue
3. Put the specimen back in its original cassette and put a lid on.
4. Put it in the with the current days run starting in formalin.
5. Process as usual.
6. embed as usual. 

You will find that the under processed part is now processed and the part
that was ok to begin with will not be harmed by the reprocessing. That part
was protected by the paraffin that had infiltrated it originally.

Good Luck.

Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
  & Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
509-954-7134
Web: www.mohshistotemp.com & www.mohslabstaffing.com 
Email: mickie25 <@t> netzero.net
 
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joe Nocito
Sent: Friday, July 27, 2007 9:20 AM
To: AGrobe2555 <@t> aol.com; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Fat issues

Albert,
could be the fat tissues didn't completely process. I read about a technique

a few years ago
(I can't remember the author,sorry) where you melt the block done, put the 
tissue between several pieces of paper towels and gently press on the tissue

to expel any remaining xylene and re-embed. I did this for a couple of colon

cases and it worked for me. It beats reprocessing the entire block.

JTT
----- Original Message ----- 
From: <AGrobe2555 <@t> aol.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, July 27, 2007 10:31 AM
Subject: [Histonet] Fat issues


> Hello All,
> I have some vascular graft tissues that we have paraffin embedded and some
> of the tissues have extra-vascular fat.  The practice sample I  sectioned 
> this
> morning seems to section well (other than the suture issues),  however the

> fat
> "blows up" as soon as I place the sections into the flotation  bath.  Is
> there some way to minimize this?  I will try cooling down  the water bath
> temperature, as it may have been a bit warm.
>
> Any suggestions are welcome.
> Thanks,
> Albert
>
> Albert C.  Grobe, PhD
> International Heart Institute of Montana Foundation
> Tissue  Engineering Lab, Saint Patrick  Hospital
>
>
>
>
>
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