[Histonet] Fat issues

Joe Nocito jnocito <@t> satx.rr.com
Fri Jul 27 11:20:05 CDT 2007

could be the fat tissues didn't completely process. I read about a technique 
a few years ago
(I can't remember the author,sorry) where you melt the block done, put the 
tissue between several pieces of paper towels and gently press on the tissue 
to expel any remaining xylene and re-embed. I did this for a couple of colon 
cases and it worked for me. It beats reprocessing the entire block.

----- Original Message ----- 
From: <AGrobe2555 <@t> aol.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, July 27, 2007 10:31 AM
Subject: [Histonet] Fat issues

> Hello All,
> I have some vascular graft tissues that we have paraffin embedded and some
> of the tissues have extra-vascular fat.  The practice sample I  sectioned 
> this
> morning seems to section well (other than the suture issues),  however the 
> fat
> "blows up" as soon as I place the sections into the flotation  bath.  Is
> there some way to minimize this?  I will try cooling down  the water bath
> temperature, as it may have been a bit warm.
> Any suggestions are welcome.
> Thanks,
> Albert
> Albert C.  Grobe, PhD
> International Heart Institute of Montana Foundation
> Tissue  Engineering Lab, Saint Patrick  Hospital
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