[Histonet] Lymph Node

[Jennifer Johnson]

I am having problems with lymph nodes.  First of all, when cutting frozens,
my Pathologist says that they are chattering too much.  When I am cutting
them, they seem to be cutting smoothly with no problem and look good on the
slide when I cover slip.  I have increased the micron, decreased the micron,
warmed for a few seconds with my thumb before cutting and they still appear
to chatter.  On the same lymph node, additional sections that were NOT
frozen are showing the same chatter as well as poor staining with
Hematoxylin.  Any help would be appreciated!

 

Thank you,

 

Jennifer Johnson

 

P.S.  In that Russian Path lab, I noticed that they had three pieces of
equipment that were newer than mine (the microtome, cryostat, and embedding
center) we just upgraded from the carousel (dip & dunk) rotary processor to
a refurbished VIP unit. Fortunately, our hospital will pay for cassettes and
we do not have to mix our reagents in old pickle jars.