[Histonet] galactosidase control

koellingr <@t> comcast.net koellingr <@t> comcast.net
Mon Jul 23 16:04:27 CDT 2007

Since you are working with transgenes in wound healing, I'm assuming this is animal work.  What I used to use for a great control was to get a mouse from (I think Jackson labs have them-but certainly one of the major mouse vendors) that is an FVB mouse with a lacZ transgene that is under control of a Tie2 promotor.  Tie2 is endothelial, so can look for beta-gal expression anywhere there is endothelial cells.  So essentially 1 mouse gives you all the controls, many tissues are fantastic, you could ever need and the staining on endothelium is specific and exquisite.

Ray Koelling
PhenoPath Laboratories
Seattle, WA
-------------- Original message -------------- 
From: "Hawkins, Hal K." <hhawkins <@t> utmb.edu> 

> We are using immunohistochemistry to detect expression of 
> beta-galactosidase from a transgene applied to healing wounds. We would 
> really like to have a good positive control. Does anyone have a block 
> they could spare for this purpose that shows selective expression of 
> galactosidase, or know of a commercial source? 
> Thanks, 
> Hal Hawkins, UTMB Galveston 
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