[Histonet] Microwave processors
JOHN P COLEMAN
JPCOLEMA <@t> sentara.com
Tue Jul 17 14:00:48 CDT 2007
I did an extensi ve validation on the Sakura Xpress. 60 watts by the
way, and what i found is that if grossing is done properly, tissue
processes well no matter the system. I have less reprocessing on Xpress
than on VIP even when pathologists gross too thick. PA's who have been
through a BS or MS program all gross appropriately. Nobody here grosses
at 1.5, and we use both the 67 and the 120 min programs depending mainly
on tissue type. Short for bxs where no gross cutting happens and for
less dense tissue and the long for most surgicals. We have a 7 hospital
system, 4 xpress processors.
John P.J. Coleman HT(ASCP)QIHC
Clinical Specialist
Anatomic Pathology
Sentara Laboratory Services
Cell/Voicemail(757)335-2159
pager: (757)456-6695
>>> <histonet-request <@t> lists.utsouthwestern.edu> 7/17/2007 12:56 PM >>>
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Today's Topics:
1. Open Positions/Pfizer, La Jolla, Ca (pam plumlee)
2. Earthquake Safety (Laurie Colbert)
3. Blood group antigen (Patti Loykasek)
4. RE: Listeria by Immunohistochemistry (Tarango, Mark)
5. Red Box Slides..Residue (Gagnon, Eric)
6. Re: Earthquake Safety (Jennifer MacDonald)
7. Methyl Gteen Counterstain (Igor Deyneko)
8. Re: Red Box Slides..Residue (Lim, David [NS])
9. Any information on the Peloris processor (jcarpenter764 <@t> aol.com)
10. Good morning from Bama! (Tammy Bailey)
11. Microwave v conventional processing (Weaver, Colin)
12. Re: Microwave v conventional processing (Rene J Buesa)
13. immunostaining for sca-1 (Melissa Mazan)
----------------------------------------------------------------------
Message: 1
Date: Mon, 16 Jul 2007 10:28:52 -0700 (PDT)
From: pam plumlee <paw555 <@t> yahoo.com>
Subject: [Histonet] Open Positions/Pfizer, La Jolla, Ca
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <837036.54887.qm <@t> web31912.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi All:
Pfizer in La Jolla, Ca has 3 full-time histology
technician positions open. In our state of the art
laboratory we do necropsy, routine research histology
and IHC (Dako and Ventana). Great learning opportunity
for the right person! Jobs to be posted on the Pfizer
website soon, until then please contact:
Pamela Plumlee H.T.
pam.plumlee <@t> pfizer.com
____________________________________________________________________________________Ready
for the edge of your seat?
Check out tonight's top picks on Yahoo! TV.
http://tv.yahoo.com/
------------------------------
Message: 2
Date: Mon, 16 Jul 2007 11:46:19 -0700
From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
Subject: [Histonet] Earthquake Safety
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<57BE698966D5C54EAE8612E8941D768301268E33 <@t> EXCHANGE3.huntingtonhospital.com>
Content-Type: text/plain; charset="iso-8859-1"
I'm interested in knowing what other people in earthquake-prone areas
do to secure their blocks and slides?
Laurie Colbert
Huntington Hospital
Pasadena, CA
------------------------------
Message: 3
Date: Mon, 16 Jul 2007 12:04:28 -0700
From: Patti Loykasek <ploykasek <@t> phenopath.com>
Subject: [Histonet] Blood group antigen
To: histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <C2C10F4C.1039E%ploykasek <@t> phenopath.com>
Content-Type: text/plain; charset="US-ASCII"
HI all. I am desperately looking for an antibody to Blood Group H that
will
work on paraffin embedded tissue. We have been using 2 different
antibodies
in the last year, and both have been discontinued (1 discontinued
after
assurances that it would not be discontinued, but that's another
story)
I would appreciate any input. Thanks.
Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA
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------------------------------
Message: 4
Date: Mon, 16 Jul 2007 13:14:46 -0700
From: "Tarango, Mark" <mtarango <@t> nvcancer.org>
Subject: RE: [Histonet] Listeria by Immunohistochemistry
To: "Charles, Roger" <rcharles <@t> state.pa.us>, "Richard Cartun"
<Rcartun <@t> harthosp.org>,
histonet <@t> lists.utsouthwestern.edu, "Marilyn
Johnson" <marjoh3 <@t> telus.net>
Message-ID:
<5AEC610C1CE02945BD63A395BA763EDE011B6D30 <@t> NVCIEXCH02.NVCI.org>
Content-Type: text/plain; charset=us-ascii
BIOCARE medical has one that works on FFPE tissue sections. Here is
the
link to the antibody datasheet. Note: OCT 4 is the same as OCT 3 and
OCT 3/4. It's a pre-diluted antibody.
http://www.biocare.net/antibodies/Oct_3_4.html
Mark Adam Tarango HT(ASCP)
Histology/Immunohistochemistry Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV 89135
mtarango <@t> nvcancer.org
Direct Line (702) 822-5112
Fax (702) 939-7663
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Charles,
Roger
Sent: Monday, July 16, 2007 5:05 AM
To: Richard Cartun; histonet <@t> lists.utsouthwestern.edu; Marilyn Johnson
Subject: RE: [Histonet] Listeria by Immunohistochemistry
In April of 2005 I ordered DIFCO's listeria O Poly Serotype 1&4
antibody
from VWR using the catalog #90001-718 with a cost of $85.00 for 1ml.
Works great on bovine brains and other species with no antigen
retrieval
needed.
Roger Charles
Microbiologist
Pennsylvania Veterinary Laboratory
2305 N Cameron St
Harrisburg, PA 17110
717-787-8808
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Richard
Cartun
Sent: Sunday, July 15, 2007 3:54 PM
To: histonet <@t> lists.utsouthwestern.edu; Marilyn Johnson
Subject: Re: [Histonet] Listeria by Immunohistochemistry
I use a polyclonal antibody that I obtained from DIFCO Laboratories
(Detroit, MI) many years ago. I don't know if it's still available.
I
would be happy to stain some unstained slides for you.
Richard
Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
(860) 545-0174 Fax
>>> "Marilyn Johnson" <marjoh3 <@t> telus.net> 07/15/07 3:20 PM >>>
Hi Histonetters,
I am looking for a primary antisera to stain for Listeria in bovine
brain.
The only source, now unavailable, was from Biodesign.
Please let me know. Thanks in advance.
Marilyn Johnson
Alberta Agriculture
Edmonton, AB. Canada
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"EMF <nvcancer.org>" made the following annotations.
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Message: 5
Date: Mon, 16 Jul 2007 16:56:03 -0400
From: "Gagnon, Eric" <gagnone <@t> KGH.KARI.NET>
Subject: [Histonet] Red Box Slides..Residue
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F93BD6329FC3AE4C8DB116B985FBC31316AB7529 <@t> KGHMAIL.KGH.ON.CA>
Content-Type: text/plain; charset="iso-8859-1"
We have been using the Red Box slides available from Newcomer Supply
for our IHC controls. These slides have a painted red box, in our case
on the back, non-frosted side of the slide. We cut the control section,
pick up the section in the red box, then dry these slides and use as
needed, picking up patient sections on the lower portion of the slide.
Recently we have encountered some patchy staining and it was suggested
by Ventana's customer service that the slides could be the cause. Their
theory is that red paint from the back of a previous slide adheres to
the front of the Red Box slide and repels reagents when they are
dispensed onto the slide, causing incomplete staining on the patient
section.
Has anyone else had recent, similar experience with these slides doing
the same thing? They are nice to use, so if there are others with the
same problem, it would definitely affect our decision to discontinue
their use and return to normal charged slides for our control cutting.
Thanks for any assistance,
Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital
Kingston, Ontario, Canada
------------------------------
Message: 6
Date: Mon, 16 Jul 2007 14:33:42 -0700
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: Re: [Histonet] Earthquake Safety
To: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OF3701CE1A.2EEDC373-ON8825731A.00764BB6-8825731A.00766116 <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"
Laurie,
At my previous place of employment we purchased locking cabinets for
the
block storage and the slide storage. We had converted to using
cardboard
file drawers for the blocks and slides for archive storage.
Jennifer
Jennifer MacDonald
Director, Histotechnician Training Program
Mt. San Antonio College
1100 N. Grand Ave.
Walnut, CA 91789
(909) 594-5611 ext. 4884
jmacdonald <@t> mtsac.edu
"Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
07/16/2007 11:46 AM
To
<histonet <@t> lists.utsouthwestern.edu>
cc
Subject
[Histonet] Earthquake Safety
I'm interested in knowing what other people in earthquake-prone areas
do
to secure their blocks and slides?
Laurie Colbert
Huntington Hospital
Pasadena, CA
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------------------------------
Message: 7
Date: Mon, 16 Jul 2007 22:41:02 -0500
From: "Igor Deyneko" <igor.deyneko <@t> gmail.com>
Subject: [Histonet] Methyl Gteen Counterstain
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<35e16a770707162041k39d84cf8tad0351c58f625acb <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Hello fellow colleagues!
I am just starting out as a new histologist and I would like to thank
everyone who responded.I recently have discovered this interesting and
useful web site and I hope to receive in future your professional
advices.
Igor Deyneko.
Infinity Pharmaceutical
Cambridge, MA.
------------------------------
Message: 8
Date: Mon, 16 Jul 2007 21:08:28 -0700
From: "Lim, David [NS]" <David.Lim <@t> vch.ca>
Subject: Re: [Histonet] Red Box Slides..Residue
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <E2D6039F5C34E04CB4F8608D453CB2570D206E <@t> vchexmb5.vch.ca>
Content-Type: text/plain; charset="iso-8859-1"
We use control etched slides for our XT; so far, no apparent issues.
The slides are manufactured by Erie and distributed through Fisher
(#22-037-228).
David Lim
Anatomic Pathology
Lions Gate Hospital
Vancouver Coastal Health
<mailto:david.lim <@t> vch.ca>
------------------------------
Message: 9
Date: Tue, 17 Jul 2007 10:17:40 -0400
From: jcarpenter764 <@t> aol.com
Subject: [Histonet] Any information on the Peloris processor
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8C996945E9F43DD-1604-4518 <@t> mblk-d15.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
Good morning Histoneers,
I thought I would post an email to see if I could get any useful
information from anyone in regards to the Peloris processor. Our lab is
interested on this specific processor, but wanted to see if we could get
any feedback, pros and/or cons regarding it. Thanks Jennell
________________________________________________________________________
AOL now offers free email to everyone. Find out more about what's free
from AOL at AOL.com.
------------------------------
Message: 10
Date: Tue, 17 Jul 2007 09:34:54 -0500
From: "Tammy Bailey" <tbailey <@t> uab.edu>
Subject: [Histonet] Good morning from Bama!
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20070717143457.31782355502 <@t> mail.vsrc.uab.edu>
Content-Type: text/plain; charset="us-ascii"
Has anyone had good luck staining with 1% phenylenediamine? I will be
staining optic nerve heads of tree shrews..Most of the papers I've
found are
from the stone age..Any help would be appreciated..
Thanks,
Tammy M. Bailey
University of Alabama-Birmingham
Vision Science Research Center
------------------------------
Message: 11
Date: Tue, 17 Jul 2007 16:17:18 +0100
From: "Weaver, Colin" <c.weaver <@t> vla.defra.gsi.gov.uk>
Subject: [Histonet] Microwave v conventional processing
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<7A885E8FE1C71C488D974EC601FAA690019E942B <@t> vla-exchn1.cvlnt.vla.gov.uk>
Content-Type: text/plain; charset="us-ascii"
Hi - we are trying to go down the microwave route in processing but
inevitably some of our veterinary pathologists are questioning whether
microwave sections are as "good" as conventional processing. Can
anyone
point me in the right direction to find any comparison done between
microwave processing and conventional overnight processing with regard
to section and staining quality.
Colin Weaver
Veterinary Laboratories Agency (VLA)
This email and any attachments is intended for the named recipient
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store or copy any of its contents and you should destroy it and inform
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Message: 12
Date: Tue, 17 Jul 2007 09:17:05 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Microwave v conventional processing
To: "Weaver, Colin" <c.weaver <@t> vla.defra.gsi.gov.uk>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <823923.82528.qm <@t> web61220.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Colin:
Using the adequate protocols MW processing renders equivalent results
to "conventional" tissue processing, that is the general concensus.
The thing is that unless you use an automated MW tissue processor, a
histotech will have to attend to the process and change reagents
manually.
This can lead to 2 problems: higher exposure of the HT to (usually
hot) chemicals and some degree of inconsistency in the protocol because
the time in each reagent could vary slightly different between runs.
Consider that a few minutes in a conventional protocol is a much
lower percentage of the time in the reagent, than the same amount of
time in a much faster protocol completed with a MW tissue processor.
MW processing should be an option when TAT is an issue and even then
there are numerous manual steps independent of the time the tissue is
involved in the processing step; they are independent of the processing
technology and usually count for the greater part of the total TAT.
Under separate cover I am sending you an article of mine wher I
analyze this issue.
RenΘ J.
"Weaver, Colin" <c.weaver <@t> vla.defra.gsi.gov.uk> wrote:
Hi - we are trying to go down the microwave route in processing but
inevitably some of our veterinary pathologists are questioning whether
microwave sections are as "good" as conventional processing. Can
anyone
point me in the right direction to find any comparison done between
microwave processing and conventional overnight processing with regard
to section and staining quality.
Colin Weaver
Veterinary Laboratories Agency (VLA)
This email and any attachments is intended for the named recipient
only.
If you have received it in error you have no authority to use,
disclose,
store or copy any of its contents and you should destroy it and inform
the sender.
Whilst this email and associated attachments will have been checked
for known viruses whilst within VLA systems we can accept no
responsibility once it has left our systems.
Communications on VLA's computer systems may be monitored and/or
recorded to secure the effective operation of the system and for other
lawful purposes.
_______________________________________________
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Message: 13
Date: Tue, 17 Jul 2007 12:38:43 -0400
From: Melissa Mazan <melissa.mazan <@t> tufts.edu>
Subject: [Histonet] immunostaining for sca-1
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <469CF093.2020506 <@t> tufts.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Hi all, we're trying to stain simultaneously for sca-1 and SPC in mouse
lung tissue - I'm unable to get the sca-1 to work (using a rat
anti-mouse clone from BD) using immunofluorescence - so am trying the
Vector ABC system. Unfortunately, to get the sca-1 to work, I have to
leave out triton-X - without the Triton-X the SPC seems not to be
working. Does anyone have advice for trying to simultaneously stain
for
an intracytoplasmic protein and a membrane protein at the same time? Is
there a better fixative than 4% formaldehyde ( we leave the tissues in
formaldehyde from 6hours to no more than 48 hours). Many thanks -
Melissa Mazan
Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor and Director of Equine Sports Medicine
Department of Clinical Sciences
Tufts Cumming School of Veterinary Medicine
200 Westborough Road
North Grafton, MA 01536
Tel:508-839-5395
Fax:508-839-7922
email: melissa.mazan <@t> tufts.edu
histonet-request <@t> lists.utsouthwestern.edu wrote:
> Send Histonet mailing list submissions to
> histonet <@t> lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
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>
> You can reach the person managing the list at
> histonet-owner <@t> lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. Milestone Pathos vs Sakura Xpress (Gregor Arlt)
> 2. RE: Milestone Pathos vs Sakura Xpress (Jes Strong)
> 3. Re: Milestone Pathos vs Sakura Xpress (Rene J Buesa)
> 4. RE: Milestone Pathos vs Sakura Xpress
> (Bartlett, Jeanine (CDC/CCID/NCZVED))
> 5. seeking hiotology position in DC area (Steven Wilkes)
> 6. Re: Vison BioSystems - Peloris (Anthony Reilly)
> 7. Hello, fellow histotechies!!! This is my first time posing a
> question - so be gentle. Our patholog (Yvonne Jones)
> 8. RE: Milestone Pathos vs Sakura Xpress
> (Bartlett, Jeanine (CDC/CCID/NCZVED))
> 9. RE: Hello, fellow histotechies!!! This is my first time
> posing a question - so be gentle. Our patholog
> (rdavis4 <@t> rdg.boehringer-ingelheim.com)
> 10. RE: Hello, fellow histotechies!!! This is my first time
> posing a question - so be gentle. Our patholog (soofia
siddiqui)
> 11. heat antigen retrieval methods (Peter Rippstein)
> 12. problems with mouse brain fixation (Martina Urbanek)
> 13. Phospho-S6 Ribosomal Protein (Goodwin, Diana)
>
>
>
----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 16 Oct 2006 14:53:33 -0500
> From: "Gregor Arlt" <histology-consultants <@t> hotmail.com>
> Subject: [Histonet] Milestone Pathos vs Sakura Xpress
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <BAY123-F138C26BAE2CF00DC277664E2090 <@t> phx.gbl>
> Content-Type: text/plain; format=flowed
>
> Dear Histonetters,
>
> I have heard the Pathos would use 600 Watts for the microwave
processing. I
> can't imagine that this is right. In my opinion this would destroy
the RNS
> structure of any tissue. On the other hand I heard tissues processed
in the
> Xpress would be easy to use for molecularbiological investigations.
>
> Has anybody experience with those instruments, or know anybody the
power of
> the microwave of the instruments.
>
> Thanks for the help Frank
>
> _________________________________________________________________
> Get FREE company branded e-mail accounts and business Web site from
> Microsoft Office Live
> http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Mon, 16 Oct 2006 15:43:55 -0500
> From: "Jes Strong" <jestrong <@t> comcast.net>
> Subject: RE: [Histonet] Milestone Pathos vs Sakura Xpress
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <00a501c6f163$cc5b0010$0200a8c0 <@t> Jes>
> Content-Type: text/plain; charset="us-ascii"
>
> Dear Mr. Arlt,
>
> If you would like to contact Milestone directly, we would be happy
to
> explain the principles of Milestone's microwave processors including
> magnetron output and how it is dynamically regulated by software to
adjust
> to each specific load. These are not questions that users would, or
should
> be expected to be able to answer for you satisfactorily.
>
> Jes Strong
> Western Region Sales Manager
> Milestone Medical
> (203) 925-4240 (Office)
> (847) 323-8373 (Cell)
> (847) 655-6009 (Fax)
> jes <@t> milestonemed.com
>
> www.milestonemed.com
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Gregor Arlt
> Sent: Monday, October 16, 2006 2:54 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Milestone Pathos vs Sakura Xpress
>
> Dear Histonetters,
>
> I have heard the Pathos would use 600 Watts for the microwave
processing. I
> can't imagine that this is right. In my opinion this would destroy
the RNS
> structure of any tissue. On the other hand I heard tissues processed
in the
> Xpress would be easy to use for molecularbiological investigations.
>
> Has anybody experience with those instruments, or know anybody the
power of
> the microwave of the instruments.
>
> Thanks for the help Frank
>
> _________________________________________________________________
> Get FREE company branded e-mail accounts and business Web site from
> Microsoft Office Live
> http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Mon, 16 Oct 2006 14:19:18 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Milestone Pathos vs Sakura Xpress
> To: Gregor Arlt <histology-consultants <@t> hotmail.com>,
> histonet <@t> lists.utsouthwestern.edu
> Message-ID: <20061016211918.92177.qmail <@t> web61214.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Frank:
> The temperature produced by the magnetron is "controlled" by the
temperature probe adjusted to the processing protocol; you can have
1200W and a working temperature of 50║C so wattage in itself is not a
deletereous agent just provides the capability of getting to high
temperatures quickly, if needed..
> Not all technologies are alike and the Xpress uses 60W continuously
in the first 2 chambers. PATHOS is pure microwave technology and Xpress
is a blend of MW and conventional technology.
> In the Xpress the first 2 chambers are identical and using MW
technology and bubble agitation. The other 2 are just 2 conventional
retorts with convection heat and vacuum capabilities, operated at 65║C.
> The molecular integrity does not relate to the process but to the
tissue fixation. Formalin greatly prevents RNA studies but any alcoholic
fixative like Kryofix, BoonFix or the propietary by Sakura (UMFix) will
preserve the macromolecules either if the tissue is going to be
processed with MW or conventional technology. Any good alcoholic
fixative containin PEG also will preserve the macromolecules.
> Both PATHOS and Xpress are "walk away" instruments but Xpress
allows for the continuous addition of up to 30 cassettes every 15
minutes, for an overall work flow of 120 cassettes after 105 minutes,
and 30 more every 15 minutes afterwards. The limit is the thickness of
the sections (have to be 1.5mm thick) and some tissues have to be
previously fixed from 4 to 4.5 hours before processing.
> PATHOS can process tissues of up to 5mm at a rate of 210
cassettes/4 hours (fixation included) for thick tissue slices or 210
cassettes/1 hour for small biopsies.
> Hope this information will help you!
> RenΘ J.
>
> Gregor Arlt <histology-consultants <@t> hotmail.com> wrote:
> Dear Histonetters,
>
> I have heard the Pathos would use 600 Watts for the microwave
processing. I
> can't imagine that this is right. In my opinion this would destroy
the RNS
> structure of any tissue. On the other hand I heard tissues processed
in the
> Xpress would be easy to use for molecularbiological investigations.
>
> Has anybody experience with those instruments, or know anybody the
power of
> the microwave of the instruments.
>
> Thanks for the help Frank
>
> _________________________________________________________________
> Get FREE company branded e-mail accounts and business Web site from
> Microsoft Office Live
> http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> All-new Yahoo! Mail - Fire up a more powerful email and get things
done faster.
>
> ------------------------------
>
> Message: 4
> Date: Mon, 16 Oct 2006 18:46:57 -0400
> From: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" <jqb7 <@t> cdc.gov>
> Subject: RE: [Histonet] Milestone Pathos vs Sakura Xpress
> To: "Gregor Arlt" <histology-consultants <@t> hotmail.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
<CB857F6460D42E4AAEA195054A25406C0ACC3378 <@t> m-ncid-2.ncid.cdc.gov>
> Content-Type: text/plain; charset="UTF-8"
>
> Frank,
>
> I have no experience with the Pathos but our lab does have the Sakura
Xpress. I am attaching a PDF of the brochure for the equipment. Page 4
explains how the microwave itself operates (60 watts). One thing I want
to add to the previous response is that the 1.5 mm thickness for the
Xpress is not mandatory. There is allowance for thicker specimens but
the processing time is then lengthened. But I for one like having an
excuse to make pathologists gross properly to begin with. :)
>
> Jeanine Bartlett
> CDC, Atlanta
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
Gregor Arlt
> Sent: Mon 10/16/2006 3:53 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Cc:
> Subject: [Histonet] Milestone Pathos vs Sakura Xpress
>
>
>
> Dear Histonetters,
>
> I have heard the Pathos would use 600 Watts for the microwave
processing. I
> can't imagine that this is right. In my opinion this would
destroy the RNS
> structure of any tissue. On the other hand I heard tissues
processed in the
> Xpress would be easy to use for molecularbiological
investigations.
>
> Has anybody experience with those instruments, or know anybody
the power of
> the microwave of the instruments.
>
> Thanks for the help Frank
>
>
_________________________________________________________________
> Get FREE company branded e-mail accounts and business Web site
from
> Microsoft Office Live
> http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 16 Oct 2006 22:15:25 -0400
> From: "Steven Wilkes" <srwilkes <@t> gmail.com>
> Subject: [Histonet] seeking hiotology position in DC area
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <8e5827cf0610161915w1f450489mb6aec23f432807ae <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> Hello
>
> I am seeking a histology position in the greater DC area. I have a
MA in
> biology, 2+ years of histology and immunohistochemistry experience,
as well
> as a bit histology teaching. Thank you
>
> Steven
>
>
> ------------------------------
>
> Message: 6
> Date: Tue, 17 Oct 2006 12:37:52 +1000
> From: "Anthony Reilly" <Tony_Reilly <@t> health.qld.gov.au>
> Subject: Re: [Histonet] Vison BioSystems - Peloris
> To: <Rcartun <@t> harthosp.org>,<histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <s534ceb6.014 <@t> health.qld.gov.au>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi Richard
>
> My peloris was part of the very first relase of the instrument. As
a
> result there were some initial minor problems which have since been
> rectified. As I said these problems were minor and never in any way
> affected the processing of our tissue.
>
> It now runs very well and has had a positive impact on our
laboratory.
> The instrument has a very powerful mixing ability which not only
> improves penetration but guarantees even heating on the steps where
heat
> is utilised giving shorter processing times even for fatty tissue.
This
> is aided by using one of their range of specimen baskets which
separates
> each cassette individually allowing better flow of solution to each
> specimen.
>
> Our laboratory services both heart and lung transplant units
requiring
> us to run numeroous short cycles throughout the day. The improved
> processing combined with a rapid clean cycle means that the one dual
> retort peloris can do the work of 3-4 of our prevoius instruments.
>
> Examples of our improved times include:
>
> Fatty tissue 18h to 14h
> Routine 12h to 9h
> Small Biopsy 2h to 1 h
>
> This has also had an impact on IHC as the small biopsies that are
> required urgently can be given an extra 1h in formalin and still be
> completed in the same time as the previous protocol. With the
faster
> processing some tissues such as lletz biopsies need to be processed
on
> shorter cycles to avoid hardening of the tissue. According to the
> manufacturer these times can be reduced further by substituting
xylene
> with isopropanol but I have not tried that so cannot comment.
>
> regards
>
>
>
> Tony Reilly
> Chief Scientist
> Anatomical Pathology
> QHPS-Prince Charles Hospital
> Rode Rd Chermside Q 4032
> Australia
> Ph: 07 3139 4543
> Fax: 07 3193 4546
> tony_reilly <@t> health.qld.gov.au
>
>
>
>>>> "Richard Cartun" <Rcartun <@t> harthosp.org> 10/15/06 11:46 pm >>>
>>>>
> Anyone out there using Vison BioSystems' "Peloris" for tissue
> processing? If so, what has been your experience? Thank you.
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-0174 Fax
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> *****************************************************************
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>
> ------------------------------
>
> Message: 7
> Date: Tue, 17 Oct 2006 02:43:21 -0400
> From: "Yvonne Jones" <yjones2 <@t> csmlab.com>
> Subject: [Histonet] Hello, fellow histotechies!!! This is my
first
> time posing a question - so be gentle. Our patholog
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <453443490200004F00000225 <@t> GWGATE1.ahm.com>
> Content-Type: text/plain; charset="US-ASCII"
>
> Hello, fellow histotechies!!! This is my first time posing a
question -
> so be gentle. Our pathologists have recently requested that we
begin
> testing an antibody, p52. I have had a bear of a time finding any
> information about this antibody!!! All I could find was one article
> on-line, and I am still having a problem finding info and the
antibody
> itself.
>
> -----------------------------------------
>
>
>
>
>
> ------------------------------
>
> Message: 8
> Date: Tue, 17 Oct 2006 05:49:06 -0400
> From: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" <jqb7 <@t> cdc.gov>
> Subject: RE: [Histonet] Milestone Pathos vs Sakura Xpress
> To: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" <jqb7 <@t> cdc.gov>, "Gregor
> Arlt" <histology-consultants <@t> hotmail.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
<CB857F6460D42E4AAEA195054A25406C11D4337E <@t> m-ncid-2.ncid.cdc.gov>
> Content-Type: text/plain; charset="us-ascii"
>
> Apparently the PDF was too large for Histonet to accept. You can go
to
> the Sakura website and click on Xpress and open the brochure if you
need
> these details.
>
> Jeanine
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Bartlett, Jeanine (CDC/CCID/NCZVED)
> Sent: Monday, October 16, 2006 6:47 PM
> To: Gregor Arlt; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Milestone Pathos vs Sakura Xpress
>
> Frank,
>
> I have no experience with the Pathos but our lab does have the
Sakura
> Xpress. I am attaching a PDF of the brochure for the equipment.
Page 4
> explains how the microwave itself operates (60 watts). One thing I
want
> to add to the previous response is that the 1.5 mm thickness for the
> Xpress is not mandatory. There is allowance for thicker specimens
but
> the processing time is then lengthened. But I for one like having an
> excuse to make pathologists gross properly to begin with. :)
>
> Jeanine Bartlett
> CDC, Atlanta
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
> Gregor Arlt
> Sent: Mon 10/16/2006 3:53 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Cc:
> Subject: [Histonet] Milestone Pathos vs Sakura Xpress
>
>
>
> Dear Histonetters,
>
> I have heard the Pathos would use 600 Watts for the microwave
> processing. I
> can't imagine that this is right. In my opinion this would
> destroy the RNS
> structure of any tissue. On the other hand I heard tissues
> processed in the
> Xpress would be easy to use for molecularbiological
> investigations.
>
> Has anybody experience with those instruments, or know anybody
> the power of
> the microwave of the instruments.
>
> Thanks for the help Frank
>
>
> _________________________________________________________________
> Get FREE company branded e-mail accounts and business Web site
> from
> Microsoft Office Live
> http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Tue, 17 Oct 2006 07:46:17 -0400
> From: rdavis4 <@t> rdg.boehringer-ingelheim.com
> Subject: RE: [Histonet] Hello, fellow histotechies!!! This is my
first
> time posing a question - so be gentle. Our patholog
> To: yjones2 <@t> csmlab.com, histonet <@t> lists.utsouthwestern.edu
> Message-ID:
>
<83BA2D3D42947D48BDAA449453644ABE0865B1 <@t> RDGEXM01.am.boehringer.com>
> Content-Type: text/plain; charset=us-ascii
>
> Yvonne,
>
> Google p52 and lots of hits come up. Also, check out www.abcam.com.
They
> have p52 as a rabbit polyclonal.
>
> Rebecca A. Davis, A.A.S., NYS LVT, HT (ASCP)
> Toxicology, Histopathology Lab
> Boehringer-Ingelheim Pharmaceuticals, Inc.
> rdavis4 <@t> rdg.boehringer-ingelheim.com
> 203-798-5448
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Yvonne Jones
> Sent: Tuesday, October 17, 2006 2:43 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Hello, fellow histotechies!!! This is my first
time
> posing a question - so be gentle. Our patholog
>
> Hello, fellow histotechies!!! This is my first time posing a
question -
> so be gentle. Our pathologists have recently requested that we
begin
> testing an antibody, p52. I have had a bear of a time finding any
> information about this antibody!!! All I could find was one article
> on-line, and I am still having a problem finding info and the
antibody
> itself.
>
> -----------------------------------------
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Tue, 17 Oct 2006 07:39:31 -0700 (PDT)
> From: soofia siddiqui <soofias2 <@t> yahoo.com>
> Subject: RE: [Histonet] Hello, fellow histotechies!!! This is
my first
> time posing a question - so be gentle. Our patholog
> To: rdavis4 <@t> rdg.boehringer-ingelheim.com, yjones2 <@t> csmlab.com,
> histonet <@t> lists.utsouthwestern.edu
> Message-ID: <20061017143931.16702.qmail <@t> web39513.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Go to Google and go check for Biocompare.com. Biocompare is a very
good source to search for any bio products. I have searched and found
all of the antibodies, that Dako has discontinued, through this web
site. Good luck!
> Soofia
>
> rdavis4 <@t> rdg.boehringer-ingelheim.com wrote:
> Yvonne,
>
> Google p52 and lots of hits come up. Also, check out www.abcam.com.
They
> have p52 as a rabbit polyclonal.
>
> Rebecca A. Davis, A.A.S., NYS LVT, HT (ASCP)
> Toxicology, Histopathology Lab
> Boehringer-Ingelheim Pharmaceuticals, Inc.
> rdavis4 <@t> rdg.boehringer-ingelheim.com
> 203-798-5448
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Yvonne Jones
> Sent: Tuesday, October 17, 2006 2:43 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Hello, fellow histotechies!!! This is my first
time
> posing a question - so be gentle. Our patholog
>
> Hello, fellow histotechies!!! This is my first time posing a question
-
> so be gentle. Our pathologists have recently requested that we begin
> testing an antibody, p52. I have had a bear of a time finding any
> information about this antibody!!! All I could find was one article
> on-line, and I am still having a problem finding info and the
antibody
> itself.
>
> -----------------------------------------
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ---------------------------------
> Get your email and more, right on the new Yahoo.com
>
> ------------------------------
>
> Message: 11
> Date: Tue, 17 Oct 2006 11:36:56 -0400
> From: "Peter Rippstein" <prippstein <@t> ottawaheart.ca>
> Subject: [Histonet] heat antigen retrieval methods
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <s534c06b.036 <@t> mail.ottawaheart.ca>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello Histonetters,
>
> Our lab is in need of some information in regards heat antigen
> retrieval methods. Has anyone done a comparison in terms of results
&
> cost effectiveness obtained from microwave irradiation vs pressure
> cooking and steam heating methods. Any recommendations would be
> appreciated. Many thanks.
> Peter
>
>
> Peter Rippstein ART, MLT
> Core Pathology Laboratory
> Rm H2102
> University of Ottawa Heart Institute
> 40 Ruskin Street
> Ottawa, Ontario
> Canada, K1Y 4W7
>
> Tel: (613) 761-5282
> Fax: (613) 761-5281
> Email: prippstein <@t> ottawaheart.ca
>
>
>
>
>
>
> -------------- next part --------------
> BEGIN:VCARD
> VERSION:2.1
> X-GWTYPE:USER
> FN:Peter Rippstein
> EMAIL;WORK;PREF;NGW:PRippstein <@t> ottawaheart.ca
> N:Rippstein;Peter
> END:VCARD
>
> BEGIN:VCARD
> VERSION:2.1
> X-GWTYPE:USER
> FN:Peter Rippstein
> EMAIL;WORK;PREF;NGW:PRippstein <@t> ottawaheart.ca
> N:Rippstein;Peter
> END:VCARD
>
> BEGIN:VCARD
> VERSION:2.1
> X-GWTYPE:USER
> FN:Peter Rippstein
> EMAIL;WORK;PREF;NGW:PRippstein <@t> ottawaheart.ca
> N:Rippstein;Peter
> END:VCARD
>
> BEGIN:VCARD
> VERSION:2.1
> X-GWTYPE:USER
> FN:Peter Rippstein
> EMAIL;WORK;PREF;NGW:PRippstein <@t> ottawaheart.ca
> N:Rippstein;Peter
> END:VCARD
>
>
> ------------------------------
>
> Message: 12
> Date: Tue, 17 Oct 2006 17:51:41 +0200
> From: Martina Urbanek <Martina.Urbanek <@t> uibk.ac.at>
> Subject: [Histonet] problems with mouse brain fixation
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <1161100301.4534fc0d23f8a <@t> web-mail1.uibk.ac.at>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hello everybody on histonet,
>
> we have some problems with handling mouse (21 and 90 days old) and
rat brains.
> We do mouse brain perfusion using 4% paraformaldehyde in PBS, pH 7.4.
We use
> gravity for perfusion for about 30 minutes (volume about 80 ml) and
after
> perfusion we leave the brains over night in the same fixative. Then
we process
> in a Shandon tissue processor (70% Alcohol, 80% Alcohol, 95% Alcohol,
3 changes
> 100% alcohol, 3 changes xylene, 2 changes paraffin 56↓C; time depends
on size
> of tissue). Now we have the problem that some brains are too hard and
some also
> seem to shrink more than others, when we cut them and put them on
waterbath
> they seem to expand and distort and often brittle. So that it looks
like only
> fibrous tissue is left, the structure is gone. I already had a look
on histonet
> archive but did not find anything that helps, therefore I hope that
someone has
> an idea what can be wrong. I have to say that we don┤t have any
problems when
> we immersion fix the brains with 4% formaldehyde.
> The problems we have with rat brains are a bit different, because we
get the
> brains from another group who perfuse the lung with 4%
paraformaldehyde in
> Hepes-buffer, pH 7.35. We then postfix over night in the same
fixative they
> use (when the brain is also perfused) or for 3 days (when brain is
not
> perfused). After dehydration in tissue processor we have the same
cutting
> problems like we observe with mouse brain tissue, even worse.
> Any suggestions are appreciated!
>
> Thank you very much for your help!!!
>
> Martina Urbanek
>
>
>
> Ms. Martina Urbanek
> Forschungslabor der
> Klin.Abt. fⁿr Neonatologie
> neonatal neuroscience research laboratory
> Med. University Innsbruck
> Innrain 66, 4th floor
> A-6020 Innsbruck
> Tel. +43 (0)512 504 27755/27765
> Fax: +43 (0)512 504 27766
> Email: Martina.Urbanek <@t> uibk.ac.at
>
>
>
>
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 13
> Date: Tue, 17 Oct 2006 12:52:24 -0400
> From: "Goodwin, Diana" <GoodwinD <@t> pahosp.com>
> Subject: [Histonet] Phospho-S6 Ribosomal Protein
> To: <HistoNet <@t> Pathology.swmed.edu>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
<80CDD9C3FEEAFD4982B114C4A6DFD00E02CB8235 <@t> uphsmbx2.UPHS.PENNHEALTH.PRV>
>
> Content-Type: text/plain; charset="us-ascii"
>
> For those of us 'Netters using this Ab on human tissue, which one and
at
> what dilution?
>
> Thanks!
>
> Diana Goodwin
> Supervisor, Anatomic Pathology
> Pennsylvania Hospital
> Preston 655-C
> ph. 215-829-6532
> pager 215-422-5160
> fax 215-829-7564
> e-mail goodwind@[pahosp.com
>
>
>
> The information contained in this e-mail message is intended only for
the personal and confidential use of the recipient(s) named above. If
the reader of this message is not the intended recipient or an agent
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please notify us immediately by e-mail, and delete the original
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>
> ------------------------------
>
> _______________________________________________
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 35, Issue 28
> ****************************************
>
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