[Histonet] c-fos staining

[Geoff McAuliffe]

Hi Jaina:

You can "paint" one side of the brain with:
1. a dilute aqueous solution (0.1% should be enough) of Alcian Blue 
OR
2. a dilute solution of aqueous picric acid.

Geoff

jaina.negandhi <@t> sickkids.ca wrote:
> Hi,
>
> I am carrying out c-fos staining on free-floating cross-sections of the
> brain. I am trying to map out the entire brain, and need to use a marker to
> distinguish between the left and right sides, as they are symmetrical.
>
> Does anyone know of a marker (eg. dye) that I can use on a full brain
> sample without causing too much damage. I'd like to be able to mark it
> without gelatin embedding if possible.
>
> Thanks,
>
> Jaina Negandhi
> Research Project Coordinator
> Neuroscience and Mental Health
> Hospital for Sick Children
> Mc Master Building, RM 3006
> Tel: 416-813-6551
> Fax: 416-813-8724
>
>
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>
>   


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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
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