[Histonet] Tracheal Whole Mounts

[John Kiernan]

Dear Ray,

Please tell us how you flattened the curved tracheal cartilages. To me, a tracheal whole-mount is just the posterior (= dorsal) wall of the trachea, which doesn't contain any cartilage. 

The tracheal  cartilages of a rat cannot be made to lie flat, and for a mouse the exercise must be (forgive the next silly phrase), even more impossible.

John Kiernan
Anatomy,  UWO
London, Canada
---
----- Original Message -----
From: koellingr <@t> comcast.net
Date: Sunday, July 15, 2007 0:12
Subject: Re: [Histonet] Tracheal Whole Mounts
To: Kim Merriam <kmerriam2003 <@t> yahoo.com>, Histonet <histonet <@t> lists.utsouthwestern.edu>

> Kim, when I did this, I got them flat first (thin pins) and then 
> immediately fixed them.  Details too lengthy.  But if 
> you really want to find out about this from someone who is an 
> expert, better than me, and that I worked with....  look up 
> Thibaut De Smedt in PubMed or Google.  He is easy to find 
> articles written by him.  He was a dendritic cell post-doc 
> where I used to work and don't know where he is now but he is 
> super nice and can give you all the info you want, far better 
> than I on this very subject.  Making the mounts and the 
> IHC.  His work was beautiful.
> 
> Ray Koelling
> Phenopath Labs
> Seattle, WA
> 
> -------------- Original message -------------- 
> From: Kim Merriam <kmerriam2003 <@t> yahoo.com> 
> 
> > Hello everyone, 
> > 
> > I have been asked to do some IHC staining on mouse tracheal 
> whole mounts. I was 
> > wondering if anyone has done this; I am looking for 
> information on how to 
> > flatten out the trachea once it is removed from the animal. 
> > 
> > Do you think I should fix it first and then flatten it or fix 
> and flatten at the 
> > same time. Any thoughts or suggestions on how to flatten a 
> mouse trachea would 
> > be greatly appreciated. 
> > 
> > Thanks, 
> > Kim 
> > 
> > Kim Merriam, MA, HT(ASCP) 
> > Cambridge, MA 
> > 
> > 
> > 
> > 
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