[Histonet] (no subject)

[Houston, Ronald]

Chunxia,

The antibody has not been shown to work on formalin-fixed paraffin embedded tissue (check the spec sheet).
My previous experience with dystroglycans and most other muscular dystrophy markers (Novocastra has the best antibodies for this type of work) is that they only work on frozen sections

Ronnie Houston, MS, HT(ASCP)QIHC
Anatomic Pathology Manager
Columbus Children's Hospital
700 Children's Drive
Columbus, OH 43205
(614) 722 5465
houstonr <@t> chi.osu.edu



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of ???
Sent: Thursday, May 24, 2007 11:26 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

  Hello, everyone! I stained frozen and paraffin-embedded sections of human skeleton and kidney tissues using the Anti-α-Dystroglycan antibody, clone VIA4-1(catalog #: 05-298) from Upstate biotechnology company by immunohistochemistry. The method is: 3um sections, EDTA 8.0 heat retrieval(pressure cooking) 10min for paraffin section，antibody dilution is 1:50,1:100 and 1:200(25 degree, 2 hours), and addition of EnVision antibody complex, and then DAB. But there was no any positive signals. 
   I do not know what is the matter. Please help me. Now, I need to know whether the dilution or reaction time is suitable. Please give me some good suggestions. I am eager to using the antibody smoothly. Thank you very much.
Sincerely,
 
Chunxia Zheng PhD
Research Institute of Nephrology, Jinling Hospital
305 East Zhongshan Road
Nanjing 210002, China
Phone: 86-25-80860218
Fax: 86-25-84801992

 
 
 


人山人海盛景，尽在梦幻西游  
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