[Histonet] Microwave processing

Douglas D Deltour doug <@t> ppspath.com
Fri Jul 13 07:47:03 CDT 2007

No offense Mr. Fisher but the information passed had to be misunderstood.
Possibly what was meant was no IHC staining should be carried out on any
microwaved processed tissues... until you internally validate it against
conventional processing. Tissue must also be validated if you are using an
alternative fixative on a conventional processor. We are using the Milestone
Pathos microwave processor which still uses 10% NBF for fixation and we are
not stuck paying for the outrageous costs of proprietary reagents. Testing
has shown no difference in IHC results.  If you are still skeptical about it
I would make a call to Ventana and speak to them about the statement that
was made in the workshop. 

Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Fax (803)254-3262
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Fischer, R.
Sent: Thursday, July 12, 2007 12:29 PM
To: Bartlett, Jeanine; John PJ Coleman; histonet <@t> lists.utsouthwestern.edu
Cc: Keating, Jeffrey - MD; Delcambre,Linda V
Subject: RE: [Histonet] Microwave processing

I have a question regarding IHC staining using tissues from a microwave
processor. I recently attended an IHC workshop at Ventana. and was surprised
to learn that IHC staining should not be carried out on any microwaved
processed tissues. I have been pro microwave processing since they hit the
marketplace, and am anxious to purchase one, but the info not to stain IHC
from one has me worried. Can anyone share info in support of this statement?
R. Brian Fischer
Histology Lead Tech
Community Hospital of the Monterey Peninsula
PO Box H.H.
Monterey, Ca. 93942
Fax: 831-625-4793


From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Bartlett,
Sent: Wed 2/22/2006 3:12 PM
To: John PJ Coleman; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Microwave processing

We have the Sakura Xpress and we are still doing side-by-side comparisons on
a variey of tissue types, IHC testing and special stains.  So far the
tissues form the Xpress have been as good as, and is some cases superior to,
the traditional processing method.

Jeanine Bartlett
CDC Atlanta

        -----Original Message-----
        From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of John PJ
        Sent: Wed 2/22/2006 5:04 PM
        To: histonet <@t> lists.utsouthwestern.edu
        Subject: [Histonet] Microwave processing

        I am the Senior tech of a large hospital corporation.  My
        administration has just won funding for 4 Sakura Microwave rapid
        processing units. We run FISH her 2 on formalin fixed paraffin
        tissue as per FDA protocol. As I tech, I am not in favor of tossing
        routine processing wholesale in favor of a completely new technology
        without thorough testing and parallel processing. Also, we are a
        regional reference lab for IHC and have a panel of 115 antibodies,
        optimized for formalin fixed paraffin embedded tissue. We run an
        average of 350 IHC slides a day, max 580 per day and would have to
        re-optimize these to use in the new formalin free world while
        our FFPE procedures in parallel for our reference lab work. Much
        running 2 labs. If anyone has any insight, or if anyone currently
        these instruments for routine and/or IHC, feel free to call or
        and I'll check the postings on this string. We are also taking
        invitations to come out and see these things in use real time.
        John PJ Coleman-757 335-2159
        Histonet mailing list
        Histonet <@t> lists.utsouthwestern.edu

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