[Histonet] Microwave processing

Thu Jul 12 14:58:09 CDT 2007

All of the microwave processors I am aware of can use formalin as well as any proprietary fixative they offer.

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Bonnie Whitaker
Sent: Thu 7/12/2007 3:50 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Microwave processing

Hi All,

I just wanted to throw my $0.02 in on this one.  I suspect that what Ventana
may have been referring to was the fixation used.  I know that at least some
of the microwave processing done out there is being done with "proprietary"
reagents and not with formalin.

  Since virtually all of the literature (as well as the IVD kits) are based
on formalin fixation for paraffin embedded tissue, then it is reasonable for
an IHC company to say that you should use formalin fixation (especially in
light of the CAP/oncologist fixation requirements for HER-2/neu).

And, for the record, I'm just playing "devil's advocate" here, not defending
Ventana in any form or fashion : )

Bonnie Whitaker

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Houston,
Ronald
Sent: Thursday, July 12, 2007 12:59 PM
To: Rene J Buesa; Fischer, R. B; Bartlett, Jeanine; John PJ Coleman;
histonet <@t> lists.utsouthwestern.edu
Cc: Keating, Jeffrey - MD; Delcambre,Linda V
Subject: RE: [Histonet] Microwave processing

I have never heard so much drivel; Ventana come out with some good ones
every so often, but this takes the biscuit.

Ronnie Houston, MS, HT(ASCP)QIHC
Anatomic Pathology Manager
Columbus Children's Hospital
700 Children's Drive
Columbus, OH 43205
(614) 722 5465
houstonr <@t> chi.osu.edu

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, July 12, 2007 1:49 PM
To: Fischer, R. B; Bartlett, Jeanine; John PJ Coleman;
histonet <@t> lists.utsouthwestern.edu
Cc: Keating, Jeffrey - MD; Delcambre,Linda V
Subject: RE: [Histonet] Microwave processing

Theoretically there should be NO difference in IHC reactions on tissue
processed using microwave technology versus "conventional" tissue
processing. You should have asked Ventana why do they "issued" that
theoretically invalid warning, what tests have they done to support it.
  René J.

"Fischer, R. B" <BFicher <@t> chomp.org> wrote:
  I have a question regarding IHC staining using tissues from a microwave
processor. I recently attended an IHC workshop at Ventana. and was surprised
to learn that IHC staining should not be carried out on any microwaved
processed tissues. I have been pro microwave processing since they hit the
marketplace, and am anxious to purchase one, but the info not to stain IHC
from one has me worried. Can anyone share info in support of this statement?
Thanks,

R. Brian Fischer
Histology Lead Tech
Community Hospital of the Monterey Peninsula
PO Box H.H.
Monterey, Ca. 93942
831-625-4791
Fax: 831-625-4793

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Bartlett,
Jeanine
Sent: Wed 2/22/2006 3:12 PM
To: John PJ Coleman; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Microwave processing

We have the Sakura Xpress and we are still doing side-by-side comparisons on
a variey of tissue types, IHC testing and special stains. So far the tissues
form the Xpress have been as good as, and is some cases superior to, the
traditional processing method.

Jeanine Bartlett
CDC Atlanta

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of John PJ Coleman
Sent: Wed 2/22/2006 5:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Cc:
Subject: [Histonet] Microwave processing

I am the Senior tech of a large hospital corporation. My administration has
just won funding for 4 Sakura Microwave rapid processing units. We run FISH
her 2 on formalin fixed paraffin embedded tissue as per FDA protocol. As I
tech, I am not in favor of tossing routine processing wholesale in favor of
a completely new technology without thorough testing and parallel
processing. Also, we are a regional reference lab for IHC and have a panel
of 115 antibodies, all optimized for formalin fixed paraffin embedded
tissue. We run an average of 350 IHC slides a day, max 580 per day and would
have to re-optimize these to use in the new formalin free world while
keeping our FFPE procedures in parallel for our reference lab work. Much
like running 2 labs. If anyone has any insight, or if anyone currently uses
these instruments for routine and/or IHC, feel free to call or email, and
I'll check the postings on this string. We are also taking invitations to
come out and see these things in use real time.

John PJ Coleman-757 335-2159 http://members.cox.net/captainmadman/
http://journals.aol.com/jcolclefa/Waytoomuchboringcraptosayusually/

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