You can try different kind of alcohol for mixing Hematoxyline. Pure ethanol is the best, but reagent alcohol also works. And don't forget to filter Hematoxylin + FeCl. Dmitriy Shtilkind, HTL (ASCP) Histology Supervisor UCI Medical Center Orange, CA, 92868 (714) 456-8731 dshtilki <@t> uci.edu