[Histonet] Microwave processing

[Fischer, R. B]

I have a question regarding IHC staining using tissues from a microwave processor. I recently attended an IHC workshop at Ventana. and was surprised to learn that IHC staining should not be carried out on any microwaved processed tissues. I have been pro microwave processing since they hit the marketplace, and am anxious to purchase one, but the info not to stain IHC from one has me worried. Can anyone share info in support of this statement?
Thanks, 
 
R. Brian Fischer
Histology Lead Tech
Community Hospital of the Monterey Peninsula
PO Box H.H.
Monterey, Ca. 93942
831-625-4791
Fax: 831-625-4793

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Bartlett, Jeanine
Sent: Wed 2/22/2006 3:12 PM
To: John PJ Coleman; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Microwave processing



We have the Sakura Xpress and we are still doing side-by-side comparisons on a variey of tissue types, IHC testing and special stains.  So far the tissues form the Xpress have been as good as, and is some cases superior to, the traditional processing method.

Jeanine Bartlett
CDC Atlanta

        -----Original Message-----
        From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of John PJ Coleman
        Sent: Wed 2/22/2006 5:04 PM
        To: histonet <@t> lists.utsouthwestern.edu
        Cc:
        Subject: [Histonet] Microwave processing
       
       

        I am the Senior tech of a large hospital corporation.  My
        administration has just won funding for 4 Sakura Microwave rapid
        processing units. We run FISH her 2 on formalin fixed paraffin embedded
        tissue as per FDA protocol. As I tech, I am not in favor of tossing
        routine processing wholesale in favor of a completely new technology
        without thorough testing and parallel processing. Also, we are a
        regional reference lab for IHC and have a panel of 115 antibodies, all
        optimized for formalin fixed paraffin embedded tissue. We run an
        average of 350 IHC slides a day, max 580 per day and would have to
        re-optimize these to use in the new formalin free world while keeping
        our FFPE procedures in parallel for our reference lab work. Much like
        running 2 labs. If anyone has any insight, or if anyone currently uses
        these instruments for routine and/or IHC, feel free to call or email,
        and I'll check the postings on this string. We are also taking
        invitations to come out and see these things in use real time.
       
        John PJ Coleman-757 335-2159
        http://members.cox.net/captainmadman/
        http://journals.aol.com/jcolclefa/Waytoomuchboringcraptosayusually/
       
       
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