[Histonet] Methods for flash freezing

Tony Henwood AnthonyH <@t> chw.edu.au
Tue Jul 10 16:50:15 CDT 2007

We use liquid notrogen, easy and very quick.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Crystal
Sent: Tuesday, 10 July 2007 9:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Methods for flash freezing

Good morning,
I am looking to get some info on flash freezing for frozen sections.
Currently we use the can freeze it spray, along with the heat extractor
in the cryostat, to do it faster, however I am concerned with safety and
feel that this poses a huge exposure risk. We have found that using the
heat extractor alone is not fast enough in cases with multiple specimens
and tend to get freeze artifact, most likely due to overzealous
pathologists. What is everyone else using? Do you use the canned spray? 
Crystal Morris M.T.(ASCP)
Anatomical Supervisor
Fairfield Medical Center Laboratory
401 N. Ewing St.
Lancaster, Ohio 43130
(740) 687-8807

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