[Histonet] Membrane label, fixable

Thomas Read tom.read <@t> utoronto.ca
Tue Jul 10 09:52:12 CDT 2007


We are trying to develop a protocol to fluorescently label the cell membrane
of live cultured HUVECs cells, to which we attach 4um fluorescent beads.
Ideally the dye would be fixable. We are using confocal microscopy and/or
deconvolution microscopy to visualize the  cell membrane/bead relationship.
So far we have tied RH414, WGA and even eosin and Evan's blue but not had
much success. Any suggestions would be greatly appreciated. Thanks very


Thomas Read, PhD
University of Toronto
Gage Institute of Research
Glaucoma Research Lab
223 College Street, Rm 308
Toronto, ON  M5T 1R4  Canada

E-mail: tom.read <@t> utoronto.ca

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