Need Dewar Flask (was) RE: [Histonet] Methods for flash freezing

Ford Royer froyer <@t>
Tue Jul 10 09:50:45 CDT 2007

I’ve been following this thread and it dawned on me that I need to buy a
Dewar flask to store liquid nitrogen, but I don’t need a new one from

Does anyone have a surplus cryogenic storage flask sitting around that they
would like to sell?

You can contact me off-List.


~ Ford

Ford M. Royer, MT(ASCP)
Minnesota Medical
7177 Madison Ave. W.
Golden Valley, MN 55427-3601
CELL:  612-839-1046
Phone:  763-542-8725
Fax:  763-546-4830
eMail:  clinicallab <@t>

-----Original Message-----
From: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] On Behalf Of Lynette
Sent: Tuesday, July 10, 2007 9:15 AM
To: Crystalmo <@t>; histonet <@t>
Subject: Re: [Histonet] Methods for flash freezing

We had very similar freezing artifacts until we purchased a Histobath
from Sakuraa few years ago.  Fits right on the counter.  You fill it
with 2-methylbutane (isopentane) and it cools it down to at least -50C. 
You occasionally have to replace it with fresh (I'd say every 3 months
or so) as it will frost up causing the temp to rise.  I wanna tell you
tho', it gives the pathologists much better nuclear
detail..........happy campers. Tissue samples freeze in less than 1
minute.  Pricing (from Cardinal Health) was around $4440 as of 1/07.
Hope this helped, 

Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI  48503

ph:  810-257-9948
fax:  810-762-7082

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