Need Dewar Flask (was) RE: [Histonet] Methods for flash freezing
froyer <@t> bitstream.net
Tue Jul 10 09:50:45 CDT 2007
Ive been following this thread and it dawned on me that I need to buy a
Dewar flask to store liquid nitrogen, but I dont need a new one from
Does anyone have a surplus cryogenic storage flask sitting around that they
would like to sell?
You can contact me off-List.
Ford M. Royer, MT(ASCP)
7177 Madison Ave. W.
Golden Valley, MN 55427-3601
eMail: clinicallab <@t> minnesotamedical.com
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lynette
Sent: Tuesday, July 10, 2007 9:15 AM
To: Crystalmo <@t> fmchealth.org; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Methods for flash freezing
We had very similar freezing artifacts until we purchased a Histobath
from Sakuraa few years ago. Fits right on the counter. You fill it
with 2-methylbutane (isopentane) and it cools it down to at least -50C.
You occasionally have to replace it with fresh (I'd say every 3 months
or so) as it will frost up causing the temp to rise. I wanna tell you
tho', it gives the pathologists much better nuclear
detail..........happy campers. Tissue samples freeze in less than 1
minute. Pricing (from Cardinal Health) was around $4440 as of 1/07.
Hope this helped,
Lynette Pavelich, HT(ASCP)
Hurley Medical Center
One Hurley Plaza
Flint, MI 48503
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