[Histonet] BUFFER

[Valeria Berno]

Hi there,

Maybe this is due only to my little experience but I am getting a little bit
confuse on the use of BUFFER!!

Someone use PBS with Ca++ and Mg++, someone else w/o, others KPBS, other PEM
other TBS.......and most of the time the answer of my question is "this is
what we have","It always worked","there is no differences",and so on....

I believe that for instance TBS is used to modify the pH, that PEM and KPBS
eliminates mild aspecific protein binding (because you have more free
charged ions?) and Ca and Mg can bind to cytoskeleton protein.

But I was wondering...what is the rationale in using one compared to another
one.

Thanks in advance for all the comments

GRAZIE

Valeria

Berno Valeria, PhD
EMBL Monterotondo Outstation
via Ramarini 32
00015 Monterotondo Scalo (RM)
Italy
Tel:  +39 06 90091 287
Fax: +39 06 90091 272
valeria.berno <@t> embl.it





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