[Histonet] Van Gieson question....

[AGrobe2555 <@t> aol.com]

Good afternoon,
We are doing an Elastic Van Gieson stain on FFPE tissues and have come up  
against a bit of a question.  While the elastin seems to be staining quite  
nicely, the nuclei either are very faintly stained or not at all.  We  have 
shortened the differentiation time in the FeCl, with little  difference. Any ideas?
Thanks, 
Albert  

Albert C.  Grobe, PhD
International Heart Institute of Montana Foundation
Tissue  Engineering Lab, Saint Patrick  Hospital





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