FW: [Histonet] ALK Protein - what do you use and how does it compare?

[Secrest, Kimberly]

Sarah,
I know Ventana offers a predilute.  Although I haven't used it yet, it will eliminate the dilution process.
Kim Secrest
WVUH

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Dana
Settembre
Sent: Tuesday, July 03, 2007 1:37 PM
To: Sarah O'Bryan; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] ALK Protein - what do you use and how does it
compare?


Hello Sarah,
I use Dako's IVD Alk Protein Cat#: M7195
I find it works well under the following conditions:
I use it at a dilution of 1:45.  I pretreat the slides with Dako's
Target Retrieval Solution
in a steamer for 40 minutes.  I incubate for 30 minutes at Room Temp
and the
detections system I use is Dako's Envision + mouse


Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA


>>> Sarah O'Bryan <sarah <@t> als.co.nz> 07/02/07 11:00 PM >>>
 

Hello all,

 

I am interested in information on ALK Protein antibodies. Who's do you
use, and how do you find it?

 

Thanks in advance,

 

Sarah O'Bryan

 

Technical Specialist

 

ALS NZ Ltd 

 

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