[Histonet] Reprocessing?

[Joe Nocito]

That might be a miss print. We melt the block down, put the tissue back in 
the block with a lid and place the block in 10% NBF. The theory is that what 
was processed the firs time, won't be processed because of the paraffin. The 
areas that did not process will go through formalin, alcohols and Xyelene. 
Then the entire block gets infiltatred with paraffin. It works pretty well 
and doesn't seem to affect immunos.

Joe
----- Original Message ----- 
From: "Wiese, Jason VHAROS" <Jason.Wiese <@t> va.gov>
To: <dcojita <@t> tampabay.rr.com>; "I-sanna Gibbons" 
<trinimaican2501 <@t> yahoo.com>; <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, July 03, 2007 5:27 PM
Subject: RE: [Histonet] Reprocessing?


So you are going from paraffin back into formalin?  I can't see how that
would possibly give you any better fixation then you already had. In
fact, I can't think of any reason at all to do this process...

JW

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
dcojita <@t> tampabay.rr.com
Sent: Tuesday, July 03, 2007 3:21 PM
To: 'I-sanna Gibbons'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Reprocessing?

I found an excellent way to reprocess tissue that does not seem to harm
it
at all.  Melt the block/tissue down, put the tissue back in the cassette
and
put the cassette lid back on.  Through the block back into paraffin and
reprocess.  No need to remove the paraffin from the tissue.  This
process is
used often in our lab and works out extremely well without harming the
tissue.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of I-sanna
Gibbons
Sent: Tuesday, July 03, 2007 11:10 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Reprocessing?

Hi all,

  One of the technicians in the lab is experiencing a problem.  He has
processed the spleen of a rat and is encountering sectioning problems -
good
paraffin ribbons but without the tissue, just the outline of where the
tissue should be.

  Is it possible for him to reverse the processing procedure,
re-process?
Will the tissue be able to withstand it?

  Thanks much

  I-sanna


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