[Histonet] Reprocessing?

[Tony Henwood]

Give it a go,
It works very well.
See: Johnson (2003) Histologic 36(1):21-22.


Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Wiese,
Jason VHAROS
Sent: Wednesday, 4 July 2007 8:28 AM
To: dcojita <@t> tampabay.rr.com; I-sanna Gibbons;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Reprocessing?


So you are going from paraffin back into formalin?  I can't see how that
would possibly give you any better fixation then you already had. In
fact, I can't think of any reason at all to do this process...

JW

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
dcojita <@t> tampabay.rr.com
Sent: Tuesday, July 03, 2007 3:21 PM
To: 'I-sanna Gibbons'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Reprocessing?

I found an excellent way to reprocess tissue that does not seem to harm
it at all.  Melt the block/tissue down, put the tissue back in the
cassette and put the cassette lid back on.  Through the block back into
paraffin and reprocess.  No need to remove the paraffin from the tissue.
This process is used often in our lab and works out extremely well
without harming the tissue.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of I-sanna
Gibbons
Sent: Tuesday, July 03, 2007 11:10 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Reprocessing?

Hi all,
   
  One of the technicians in the lab is experiencing a problem.  He has
processed the spleen of a rat and is encountering sectioning problems -
good paraffin ribbons but without the tissue, just the outline of where
the tissue should be.
   
  Is it possible for him to reverse the processing procedure,
re-process? Will the tissue be able to withstand it?
   
  Thanks much
   
  I-sanna

       
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