[Histonet] vacuum impregnation shedule

[I-sanna Gibbons]

Hi everyone,
  I'm processing the brainstem of a bat (about 7-10mm long, 3mm thick) using a double impregnation method.  The paraffin wax stage comprises 4 changes of wax, 9 hours total duration.  However, I would like to use 2 wax changes in a vacuum at this stage but am not sure of how to modify/ decrease the duration.  Also, what pressure should the vacuum be set at?
  Thanks again
  I-sanna

       
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