[Histonet] Reprocessing?

[Derek Papalegis]

I-sanna Gibbons wrote:
> Hi all,
>    
>   One of the technicians in the lab is experiencing a problem.  He has processed the spleen of a rat and is encountering sectioning problems - good paraffin ribbons but without the tissue, just the outline of where the tissue should be.
>    
>   Is it possible for him to reverse the processing procedure, re-process?  Will the tissue be able to withstand it?
>    
>   Thanks much
>    
>   I-sanna
>
>        
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This is a common occurance with rodent tissue in general. It is because 
the tissue is so lean. Put a paper towel on the cold tray and soak it 
with water and let the tissue sit on there for 20-30 minutes then try 
cutting again. It should cut no problem then. If you have to take 
multiple sections and go deep into the block, you might have to do this 
a few times. If that doesn't work, put the block in the heated water 
bath for a minute or 2 then put back on the cold tray. That should help 
as well.

-Derek

-- 
Derek Papalegis HT (ASCP)
Histotechnician
Division of Laboratory Animal Medicine
Tufts University 
136 Harrison Avenue
Boston, MA 02111
phone: 617 636-2971
fax: 617 636-8354