[Histonet] Nuclear artefact II
Kemlo.Rogerson <@t> waht.swest.nhs.uk
Mon Jul 2 07:48:26 CDT 2007
The FS was doused in aceto-alcohol as per usual.
The pleural fluid was a cytospin (how I now hate that device) fixed in
alcohol in our normal way. (It is as you observe no doubt, air dried at
the edge from which I took the photo, but that's because the bubbles
show better there).
Now - there's the basis of a separate thread - how can you tell whether
a smear is fixed well or not? What is "fixed well"?
So it dried before it was fixed? My point exactly and that's why the
bubbles show better!!!
"Fixed well" I assume means that the fixative has either attached itself
completely to all those things it ought to attach itself to, altered the
tertiary protein structures sufficiently to trap all the substances that
are able to be trapped and rendered the matrix of the tissue's proteins
sufficiently robust to withstand processing and exposed all those sites
needing exposing to facilitate staining then I assume it is fixed
Cytospins are, IMHO, the Devils tool and introduces artefacts and loses
cells as you suggest; LBC is the way forward and I assume that means
Cytyc? Has SurePath cracked the LBC world for Non-Gynae yet? As a
recovering Cell Path Manager I'm losing touch, but oddly Pharmacies and
the therapies are fun too.
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