[Histonet] Used Microscopes: Attn Vendors

Tue Jan 30 10:42:27 CST 2007

Hi,

  We have a client interested in purchasing a used microscope with a miller disc.  If you have any used scopes available, please fax your quotes to 706-546-6522.

  Thanks so much.

  Louri Roberts

  Billing Consultants, LLC
  www.billingconsultants.net

histonet-request <@t> lists.utsouthwestern.edu wrote:
  Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu

You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."

Today's Topics:

1. RE: Xylene substitute (sheila adey)
2. Re: Xylene substitute (Lynette Pavelich)
3. RE: pens (Bonner, Janet)
4. Miller's Elastic Stain (Andrea Grantham)
5. Hypoxia (Gamal Akabani)
6. RE: Miller's Elastic Stain (Monfils, Paul)
7. If you are even remotely curious, please call! Even more temp
openings-- (Cheryl Kerry)
8. Re: Miller's Elastic Stain (Bryan Hewlett)
9. Re: Miller's Elastic Stain (Bryan Llewellyn)
10. Re: Miller's Elastic Stain (Andrea Grantham)
11. Re: Anybody doing flow cytometry? What does Neat mean?
(Christina Thurby)
12. ASCP Exam (Stasha McCollum)
13. Processing debate (GMartin <@t> marshallhospital.org)
14. a question (nizar limaiem)
15. Re: ASCP Exam (Kaye Ryan)
16. human total IgG (Liz Chlipala)
17. RE: Re: Anybody doing flow cytometry? What does Neat mean?
(Monfils, Paul)
18. MCP1 positive control (Brown, Graham W)
19. Re: Mouse Testes Fixation and Processing (Laurie Reilly)
20. Re: Immunogold question (Robert Chiovetti)
21. Follow up (GMartin <@t> marshallhospital.org)
22. RE: Re: Anybody doing flow cytometry? What does Neat mean?
(Tarango, Mark)
23. ASCP EXAM (MICHELLE SEAGLE)
24. Re: Hypoxia (Jakub Otahal)
25. RUO's and diagnostic IHC (Houston, Ronald)
26. Re: Processing debate (Rene J Buesa)

----------------------------------------------------------------------

Message: 1
Date: Mon, 29 Jan 2007 13:38:44 -0500
From: "sheila adey" 
Subject: RE: [Histonet] Xylene substitute
To: GMartin <@t> marshallhospital.org, histonet <@t> lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; format=flowed

We tried using formula 88 and found it to be also irritating, especially 
after a spill. It was a problem when we tried to coverslip because our 
sugipath mounting media is Xylene based but since you have already covered 
that issue, it would be nice to hear what you think of the quality of the 
product. Please share after you've been using it.

Sheila Adey HT MLT
Port Huron Hospital
Michigan

>From: GMartin <@t> marshallhospital.org
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Xylene substitute
>Date: Mon, 29 Jan 2007 09:15:00 -0800
>
>We presently use Xylene in our processor. We will be moving to a Xylene
>substitute. We are a small lab and still hand cover all of our slide. In
>the cover slip line we do use Sugipath's Sub-x Xylene substitute and are
>planning on using Sub-x.
>Can anyone in the group fill me in on their experience with a change over
>to a Xylene substitute.
>Thank you
>Gary Martin
>El Dorado pathology
>California.
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_________________________________________________________________
Your Space. Your Friends. Your Stories. Share your world with Windows Live 
Spaces. http://discoverspaces.live.com/?loc=en-CA

------------------------------

Message: 2
Date: Mon, 29 Jan 2007 13:41:25 -0500
From: "Lynette Pavelich" 
Subject: Re: [Histonet] Xylene substitute
To: ,
Message-ID: <45BDF985020000EE00010CF6 <@t> smtp-gw.hurleymc.com>
Content-Type: text/plain; charset=US-ASCII

Hi Gary,
We recenty had a change over to a substitute and went with the aliphatic
Propar by Anatech. Easy transition, very low oder, docs didn't notice
(big plus). We did add an extra container on the stainer when
depariffinizing, and on the processor, we have 3 containers of the
substitute. We are also able to recycle it. Works well with hand
coverslipping media also. You'll be happy with the change.
Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI 48503

ph: 810-257-9948
fax: 810-762-7082
>>> 01/29/07 12:15 PM >>>
We presently use Xylene in our processor. We will be moving to a Xylene
substitute. We are a small lab and still hand cover all of our slide. 
In
the cover slip line we do use Sugipath's Sub-x Xylene substitute and are
planning on using Sub-x.
Can anyone in the group fill me in on their experience with a change
over
to a Xylene substitute.
Thank you
Gary Martin
El Dorado pathology
California.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 3
Date: Mon, 29 Jan 2007 14:51:14 -0500
From: "Bonner, Janet" 
Subject: RE: [Histonet] pens
To: "Patricia Karlisch" 
,
Histonet <@t> lists.utsouthwestern.edu, "ruegg, patsy"

, "Webb', 'Dorothy L"

Message-ID:
<5F31F38C96781A4FBE3196EBC22D478004A73C <@t> fhosxchmb006.ADVENTISTCORP.NET>

Content-Type: text/plain; charset=iso-8859-1

We have been looking for a similar pen that will stay on the cassettes AND the slides. Right now we use two different pens. Smudging has been a problem, but the Mercedes pens seem like a winner!
Janet

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Patricia Karlisch
Sent: Mon 1/29/2007 9:18 AM
To: Histonet <@t> lists.utsouthwestern.edu; ruegg, patsy; Webb', 'Dorothy L
Subject: RE: [Histonet] pens

All,
I have received good recommendations for cassette pens and it looks like there are many more pens out there then I knew about. We will try each one and see if this alleviates the smudging. Thank you all so much. Pat Karlisch.

Pat Karlisch
Supervisor, Histology, Pathology and Laboratory Medicine
Penn State Milton S. Hershey Medical Center
Mail Code H179
Hershey, PA 17033
Phone (717) 531-6072
Fax: (717) 531- 7741
email: pkarlisch <@t> psu.edu

*****E-Mail Confidentiality Notice*****
This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately.

>>> "patsy ruegg" 
1/27/2007 7:44 PM >>>

We use pens from StatLabs we like, just have to let the cassettes dry a
minute or two before putting them in alcohol waiting for processing.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patricia
Karlisch
Sent: Wednesday, January 24, 2007 8:54 AM
To: Dorothy L Webb; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] pens

Dorothy,
Thank you for this information. We are looking for non-smudge pens
as well. This will help. Did you find that the Surgipath pens did not
smudge or come off during processing?

Pat Karlisch
Supervisor, Histology, Pathology and Laboratory Medicine
Penn State Milton S. Hershey Medical Center
Mail Code H179
Hershey, PA 17033
Phone (717) 531-6072
Fax: (717) 531- 7741
email: pkarlisch <@t> psu.edu

*****E-Mail Confidentiality Notice*****
This message (including any attachments) contains information intended
for a specific individual(s) and purpose that may be privileged,
confidential or otherwise protected from disclosure pursuant to
applicable law. Any inappropriate use, distribution or copying of the
message is strictly prohibited and may subject you to criminal or civil
penalty. If you have received this transmission in error, please reply
to the sender indicating this error and delete the transmission from
your system immediately.

>>> "Webb, Dorothy L" 1/24/2007
10:49 AM >>>

We recently had the same problems and researched several companies and
brands and found for our usage the following were the best:

Surgipath pens #01880 come in a box of 10 for $27.00

Marketlab pens #ML0479 come in a box of 10 for $37.00

Mercedes Medical has a new pen from Norway that we tried that are only
$13 per box of 10, but do not have the order number for them as they
are
not in stock quite yet.

The pens from Newcomer (Histo pen) and the Cancer Diagnostics pen did
not smear, etc., but dried out so fast and often came already dry in
the
box.

Good Luck!!
________________________________________
This e-mail and any files transmitted with it are confidential and are
intended solely for the use of the individual or entity to whom they are
addressed. If you are not the intended recipient or the individual
responsible for delivering the e-mail to the intended recipient, please
be advised that you have received this e-mail in error and that any use,
dissemination, forwarding, printing, or copying of this e-mail is
strictly prohibited.

If you have received this e-mail in error, please immediately notify
the HealthPartners Support Center by telephone at (952) 967-6600. You
will be reimbursed for reasonable costs incurred in notifying us.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

=======================================================
The information contained in this message may be privileged and/or confidential
and protected from disclosure. If the reader of this message is not the intended 
recipient or an employee or agent responsible for delivering this message to the 
intended recipient, you are hereby notified that any dissemination, distribution 
or copying of this communication is strictly prohibited. If you have received this
communication in error, please notify the sender immediately by replying to this 
message and deleting the material from any computer.
=======================================================

------------------------------

Message: 4
Date: Mon, 29 Jan 2007 13:33:12 -0700
From: Andrea Grantham 
Subject: [Histonet] Miller's Elastic Stain
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<4.3.2.7.2.20070129130923.00e1fec0 <@t> algranth.inbox.email.arizona.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

Happy Monday!
I have had a request to do a Miller's Elastic Stain. I have almost all of 
the stains and I do have all of the chemicals to do the stain but I have a 
question.
One of the ingredients in the elastic stain is resorcin. I'm not a chemist 
so I have to ask this question, I have resorcinol in my chemical cabinet - 
is this the same thing as resorcin? I can find resorcinol in the Sigma cat. 
but not resorcin.

Thanks!
Andi Grantham
.....................................................................
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
:...................................................................:
http://www.cba.arizona.edu/histology-lab.html

------------------------------

Message: 5
Date: Mon, 29 Jan 2007 14:40:39 -0600
From: Gamal Akabani 
Subject: [Histonet] Hypoxia
To: "Histonet ((E-mail))" 
Message-ID: <4885123C-E70A-41BC-AAE0-1F483B088D7A <@t> gmail.com>
Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed

Dear friends,

I am want to process some histological samples from glioma patients 
in order to observe the level or grade of hypoxia on them, if any.
I am not a guru. Any help is appreciated on the methods or a 
specific antibody used to visualize it. Thanks in advanced.

Gamal

------------------------------

Message: 6
Date: Mon, 29 Jan 2007 15:50:40 -0500
From: "Monfils, Paul" 

Subject: RE: [Histonet] Miller's Elastic Stain
To: 
Message-ID:
<4EBFF65383B74D49995298C4976D1D5E273C0E <@t> LSRIEXCH1.lsmaster.lifespan.org>

Content-Type: text/plain; charset="iso-8859-1"

Yes, resorcinol and resorcin are the same thing, C6H6O2

------------------------------

Message: 7
Date: Mon, 29 Jan 2007 14:53:02 -0600
From: "Cheryl Kerry" 
Subject: [Histonet] If you are even remotely curious, please call!
Even more temp openings--
To: 
Message-ID: <00b501c743e7$77b140c0$6401a8c0 <@t> FSDESKTOP>
Content-Type: text/plain; charset="US-ASCII"

Hi All!

We're swamped and need more great techs!! If you have ever been ever
remotely curious about travel temps, I would love to talk with you. Even if
you won't be ready for a year or more--let's start the conversation so when
you are ready to go, you're comfortable and happy in your decision. 

I keep in touch. I don't pressure you (it is YOUR life, right?) Someone said
I don't come across as a used car salesman.I'm a histotech!! If something
doesn't fit--you tell me 'no' and we move on to the next opening. Easy as
that. 

I have EIGHT open positions and need to fill them within the next two weeks.
All shifts, all skills. With the changes in the registry and state
licensing, we're going to continue to need great techs who want to try new
things. 

Give me a call--it's an investment of about 15 minutes for the basics. I
help new travelers get started and we've got a great team. It all starts
with a question- "So, tell me about temping?" 

I'll give you the details of the jobs that fit when we get a chance to
chat!! 

Cheryl :-)

Cheryl Kerry, HT(ASCP)
Full Staff Inc

281.852.9457

800.756.3309

------------------------------

Message: 8
Date: Mon, 29 Jan 2007 15:54:58 -0500
From: "Bryan Hewlett" 
Subject: Re: [Histonet] Miller's Elastic Stain
To: , "Andrea Grantham"

Message-ID: <000d01c743e7$bd2f0470$6500a8c0 <@t> mainbox>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=response

Andi,

Resorcin is the same thing as resorcinol!

Bryan

----- Original Message ----- 
From: "Andrea Grantham" 
To: 
Sent: Monday, January 29, 2007 3:33 PM
Subject: [Histonet] Miller's Elastic Stain

> Happy Monday!
> I have had a request to do a Miller's Elastic Stain. I have almost all of 
> the stains and I do have all of the chemicals to do the stain but I have a 
> question.
> One of the ingredients in the elastic stain is resorcin. I'm not a chemist 
> so I have to ask this question, I have resorcinol in my chemical cabinet - 
> is this the same thing as resorcin? I can find resorcinol in the Sigma 
> cat. but not resorcin.
>
> Thanks!
> Andi Grantham
> .....................................................................
> : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
> : Sr. Research Specialist University of Arizona :
> : (office: AHSC 4212) P.O. Box 245044 :
> : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
> : (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
> :...................................................................:
> http://www.cba.arizona.edu/histology-lab.html
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

------------------------------

Message: 9
Date: Mon, 29 Jan 2007 12:43:26 -0800
From: Bryan Llewellyn 
Subject: Re: [Histonet] Miller's Elastic Stain
To: Histonet , Andrea Grantham

Message-ID: <001201c743e6$209f0520$6400a8c0 <@t> yourlk4rlmsu>
Content-Type: text/plain; format=flowed; charset=iso-8859-1;
reply-type=response

Same thing.

Bryan Llewellyn

----- Original Message ----- 
From: "Andrea Grantham" 
To: 
Sent: Monday, January 29, 2007 12:33 PM
Subject: [Histonet] Miller's Elastic Stain

> Happy Monday!
> I have had a request to do a Miller's Elastic Stain. I have almost all of 
> the stains and I do have all of the chemicals to do the stain but I have a 
> question.
> One of the ingredients in the elastic stain is resorcin. I'm not a chemist 
> so I have to ask this question, I have resorcinol in my chemical cabinet - 
> is this the same thing as resorcin? I can find resorcinol in the Sigma 
> cat. but not resorcin.
>
> Thanks!
> Andi Grantham
> .....................................................................
> : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
> : Sr. Research Specialist University of Arizona :
> : (office: AHSC 4212) P.O. Box 245044 :
> : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
> : (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
> :...................................................................:
> http://www.cba.arizona.edu/histology-lab.html
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

------------------------------

Message: 10
Date: Mon, 29 Jan 2007 14:19:22 -0700
From: Andrea Grantham 
Subject: Re: [Histonet] Miller's Elastic Stain
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<4.3.2.7.2.20070129141822.00e1a5e0 <@t> algranth.inbox.email.arizona.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

Thanks! Glad to hear that it is the same thing. Looks like I'm good to go.
Andi

At 01:33 PM 1/29/2007 -0700, Andrea Grantham wrote:
>Happy Monday!
>I have had a request to do a Miller's Elastic Stain. I have almost all of 
>the stains and I do have all of the chemicals to do the stain but I have a 
>question.
>One of the ingredients in the elastic stain is resorcin. I'm not a chemist 
>so I have to ask this question, I have resorcinol in my chemical cabinet - 
>is this the same thing as resorcin? I can find resorcinol in the Sigma 
>cat. but not resorcin.
>
>Thanks!
>Andi Grantham
>.....................................................................
>: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
>: Sr. Research Specialist University of Arizona :
>: (office: AHSC 4212) P.O. Box 245044 :
>: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
>: (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
>:...................................................................:
> http://www.cba.arizona.edu/histology-lab.html
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

.....................................................................
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :

=== message truncated ===

---------------------------------
No need to miss a message. Get email on-the-go 
with Yahoo! Mail for Mobile. Get started.