[Histonet] Processing debate

Cheri Miller cmiller <@t> physlab.com
Tue Jan 30 10:09:54 CST 2007

We place our cassettes in numerical order. We also use s different color
cassette for every 20 cases. Seemed silly when I first got here, Now I rely
on those colors to guide me to what comes next. We also use a certain color
cassette for STATS and ASAP cases. It's like a" heads up people!" this case
needs extra attention.

Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, January 30, 2007 9:03 AM
To: GMartin <@t> marshallhospital.org; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Processing debate

Fear the word "random", in histology is almost synonym with "caos".There is
absolutely no logic or benefit from placing the cassettes at random.
  If you use a cassetting log the sequencial and orderly manner of placing
the cassettes following the log order is the way of doing things.
  You can always know where your cassettes are and at the embedding step you
can follow the same sequency and easily determine if all the cassettes were
processed and embeded.
  It is absolutely illogical to place anything at random (unless you are
selecting something for an experimental design that requires that type of
  The only "logical" explanation to your initial question is that it stems
from the pathologist's lazyness that prefers to throw the cassettes in the
container and letting the histotech to try to organize what should not have
been disorganized in the first place.
René J.

GMartin <@t> marshallhospital.org wrote:
  We presently process with a Tissue Tek Vip 2000. Our pathologist cut
the tissue in. the debate is this ... one side says that the cassettes
must be placed in a container of formalin to float at random before being
placed into the Vip 2000 basket.
The other side of the debate says that the tissue cassettes can be
placed in order directly into the basket that is submerged in formalin.
Can the group shine any light on this so far civil debate :)

Gary Martin
El Dorado Pathology

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