[Histonet] bone softening

DiCarlo, Margaret MDiCarlo <@t> KaleidaHealth.Org
Tue Jan 30 09:58:00 CST 2007



A while ago I remember someone mentioning a method to soften bone after
it's been decaled but prior to processing.  Can anyone tell me the
procedure and does it have any affect on the H&E staining?  Lately, my 5
x 7" size bones have been giving me great difficulty when sectioning
since they have been very dense and hard.   I process with xylene.  


Thanks for your help.


Peggy DiCarlo HT (ASCP)

Orthopedics Bone Lab

Buffalo General Hospital

100 High St.

Buffalo, NY  14203



Kaleida Health's economic impact on Western NY exceeds $2.2 BILLION annually.

Check us out at: www.WesternNYshospitalofchoice.org

This email transmission and any documents, files, 
or previous e-mail messages attached to it are 
confidential and intended solely for the use of the 
individual or entity to whom they are addressed. 
If you are not the intended recipient, or a person 
responsible for delivering it to the intended recipient, 
you are hereby notified that any further review, 
disclosure, copying, dissemination, distribution, or 
use of any of the information contained in or attached 
to this e-mail transmission is strictly prohibited. 
If you have received this message in error, please 
notify the sender immediately by e-mail, discard 
any paper copies, and delete all electronic files 
of the message. If you are unable to contact the 
sender or you are not sure as to whether you 
are the intended recipient, please e-mail 
ISTSEC <@t> KaleidaHealth.org or call (716) 859-7777.

More information about the Histonet mailing list