abright <@t> brightinstruments.com
Thu Jan 25 10:15:53 CST 2007
On our cryostats I would have the specimen temperature set @ -12ºC and the microtome chamber temperature set @ -20ºC as this will stop any adherence to the knife and anti-roll plate. You are much too cold at present on the specimen temperature.
I see you are a neuro lab these settings would be fine for brain too.
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From: Inga Hansson [mailto:Inga.Hansson <@t> neuro.uu.se]
Sent: 25 January 2007 12:54
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] testicles
A collegue of mine is trying to section frozen rat testicle (perfused
and cryoprotected in sucrose) at -30°C. Is that a good temperature to
use? The sections seem fragile and tend to break. They´re 10 microns
thick. Or is it bad fixation?
Thanks in advance for any help!
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