[Histonet] testicles

[Alan Bright]

Dear Inga,

On our cryostats I would have the specimen temperature set @ -12ºC and the microtome chamber temperature set @ -20ºC as this will stop any adherence to the knife and anti-roll plate. You are much too cold at present on the specimen temperature. 
I see you are a neuro lab these settings would be fine for brain too.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright <@t> brightinstruments.com
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-----Original Message-----
From: Inga Hansson [mailto:Inga.Hansson <@t> neuro.uu.se] 
Sent: 25 January 2007 12:54
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] testicles


Hi everyone,

A collegue of mine is trying to section frozen rat testicle (perfused 
and cryoprotected in sucrose) at -30°C. Is that a good temperature to 
use? The sections seem fragile and tend to break. They´re 10 microns 
thick. Or is it bad fixation?

Thanks in advance for any help!

Inga


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