[Histonet] embedding without a station?

[Blazek, Linda]

Someone needs to teach Anila how to make a boat!

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: lblazek <@t> digestivespecialists.com

 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Anila
Syed
Sent: Wednesday, January 24, 2007 1:12 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] embedding without a station?

Wow!! Thanks for all the suggestions and trips down memory lane! I dont
get
how paper could have been used as moulds!! and I love the teapot idea!!
As
for the asbestos and lead etc, I won't mention any of that to our safety
officer :)

Thanks everyone, I will try to go with a beaker in the incubator and a
hotplate, but will also try the doughnuts approach :)

Thanks

Anila
----- Original Message ----- 
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, January 24, 2007 4:50 PM
Subject: RE: [Histonet] embedding without a station?


We didn't have embedding stations when I started in histology. We had
three
stainless steel pitchers of melted paraffin, maybe 500 ml each, in the
paraffin oven. We worked on a large, low temperature hotplate, called a
"slide warmer".  We would take one of the pitchers to the "work
station",
place it on the slide warmer.  We would take the last paraffin
container,
containing the processed specimens, off the rotary processor and plug it
in
next to the hotplate. Take an embedding mold (these were made of paper,
folded by the histotechs at the end of the previous workday), write the
specimen ID on it, place it on the hotplate, pour paraffin into it from
the
pitcher, unwrap the tissue sample (which were processed wrapped in lens
paper - no such thing as cassettes), place it in the mold, then transfer
the
mold to a metal plate on top of a cakepan full of ice.  When the pitcher
of
paraffin started to get too cool, replace it in the oven and take
another
pitcher. Sounds pretty primitive - mainly because it was - but it got
the
job done.

> ----------
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Anila
Syed
> Sent: Wednesday, January 24, 2007 5:05 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] embedding without a station?
>
> Dear All,
>
> I have hundreds of carotid plaques to embedd. I have a tissue
processor,
but
> no embedding station. Would anyone attempt to do this without an
embedding
> station or do you think I should go and try to find the facilities
> somewhere?
>
> What did people do before embedding stations?
>
> Many thanks for your input and opinions,
>
> Anila Syed
>
>
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