[Histonet] RE: Dendritic cells
Meilus, Sheri D.
Sheri.Meilus <@t> va.gov
Mon Jan 22 10:50:16 CST 2007
Hi Melissa,
Have you tried S-100 to stain the dendritic cells? Depending on their
lineage, dendritic cells are immunoreactive with S-100 protein.
Sheri
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
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Sent: Saturday, January 20, 2007 1:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 38, Issue 30
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Today's Topics:
1. FNA Billing (Kelly Larson)
2. Spam not related to Histonet but is vendor related (Gayle Callis)
3. Paraffin sectioning troubleshooting (AGrobe2555 <@t> aol.com)
4. Double staining of 2 antibodies with 2 different retrieval
methods (Patrick Laurie)
5. Embedding Station (Helen E Johnson)
6. Date of Service (Richard Cartun)
7. RE: Date of Service (Weems, Joyce)
8. mouse DC marker on FFPE? (Melissa Gonzalez)
9. QC/QA IHC position open at ThermoFisher Scientific - Lab
Vision, California (Morken, Tim)
10. Software needed for Shurmark cassette/slide printer
(Jackie M O'Connor)
11. RE: Date of Service (Tarango, Mark)
12. Collaboration Solutions for Clinical Labs and BioPharma
(jayant limaye)
----------------------------------------------------------------------
Message: 1
Date: Fri, 19 Jan 2007 13:08:53 -0500
From: "Kelly Larson" <kfineout <@t> hotmail.com>
Subject: [Histonet] FNA Billing
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY130-F29E9A1CEC8AB770A6A89EBCEA90 <@t> phx.gbl>
Content-Type: text/plain; format="flowed"
I'm wondering what CPT codes you can bill when receiving an FNA
into
your lab and making Thinprep slides for your pathologist
to
interpret??
Thanks for your input.
Kelly
_________________________________________________________________
[1]Get FREE Web site and company branded e-mail from Microsoft
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References
1. http://g.msn.com/8HMAENUS/2734??PS=47575
------------------------------
Message: 2
Date: Fri, 19 Jan 2007 11:20:48 -0700
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] Spam not related to Histonet but is vendor related
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20070119111136.01b3c5d8 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Dear Histonetters,
Have you been hit with spam messaging about the new Ventana Pathway,
whatever? I have just been spammed, totally unrelated to Histonet for
investing, or whatever in Ventana, blah, blah, blah.
Being a busy, but TGIF and hating spam with a passion - this really
hacked
me off big time! Ventana should know better than to allow whoever, or
whatever to spam in this willy nilly junk fashion. I would rather have
them contact me directly than see them join in with the other boring,
excessive spammers.
Hopefully, they are looking in on this email and then change their
spammy
ways. I would be happy to discuss this with them one on one at any
time.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
------------------------------
Message: 3
Date: Fri, 19 Jan 2007 13:23:22 EST
From: AGrobe2555 <@t> aol.com
Subject: [Histonet] Paraffin sectioning troubleshooting
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <c44.cac9005.32e2669a <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
How thick are your sections? Have you tried adjusting your knife
angle? I
keep my blocks in the refrigerator the day I am sectioning, and take
them out
just before sectioning. I allow them to warm for a few minutes, and
ribbon
off my sections. If I start having problems, I put a frozen artificial
ice
pack on the block surface for 30-60 seconds, allow to warm for a few
seconds,
then section again.
The brittleness of your sections sounds like a processing issue. You
may
need to adjust your processing schedule if the tissues are brittle,
especially
if you are using an automatic processor. If the wax is brittle, try
another
wax formulation with additional plasticizers that serve to "soften" the
wax.
------------------------------
Message: 4
Date: Fri, 19 Jan 2007 11:20:44 -0800
From: "Patrick Laurie" <plaurie <@t> benaroyaresearch.org>
Subject: [Histonet] Double staining of 2 antibodies with 2 different
retrieval methods
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4f7b0fea590ccd4c8d23b73df6d05f14 <@t> localhost.localdomain>
Content-Type: text/plain; charset="us-ascii"
Hello Histonet,
I am trying to do some double labeling of ubiquitin and HLA-DR LN3
antibody on human spinal cord. My PI wants to be able to see both the
ubiquitinization of the motor neurons with patients that had ALS, as
well as seeing the proliferation and activation of microglia on the same
slide, near the same cell. But, I have found that the ubiquitin I am
using, (Chemicon's Monoclonal ubiquitin antibody) requires no antigen
retrieval (AR), while the HLA-DR LN3 antibody (monoclonal from MP
biomedical) works best with a 30 min citrate retrieval. The citrate
retrieval with the Ubiquitin creates too much background staining,
blocking reagents are powerless to stop it. Sooo, to make a long story
short, what would be the easiest way to get my PI's (not my) desired
result? He wants to stay with chemicons monoclonal ubiquitin, Dako's is
a polyclonal, but it also doesn't want AR. Does anyone have any
suggestions, is this a hopeless cause?
Thanks in advance for your advice,
Patrick Laurie, HT (ASCP)
Neurogenomics Laboratory
Benaroya Research Institute
1201 9th Ave
Seattle, Wa 98101
(206) 341-0681
------------------------------
Message: 5
Date: Fri, 19 Jan 2007 14:32:42 -0500
From: Helen E Johnson <hej01 <@t> health.state.ny.us>
Subject: [Histonet] Embedding Station
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFC3DB7127.74776168-ON85257268.006B1C35-85257268.006B5E72 <@t> notes.health.
state.ny.us>
Content-Type: text/plain; charset=US-ASCII
Hi histonetters,
Our lab would like to purchase a new embedding station. What
would
you recommend?
Helen Johnson
(hej01 <@t> health.state.ny.us)
------------------------------
Message: 6
Date: Fri, 19 Jan 2007 14:36:35 -0500
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: [Histonet] Date of Service
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <45B0D7730200007700003D8B <@t> hcnwgwds01.hh.chs>
Content-Type: text/plain; charset=US-ASCII
When you receive a consult from another institution, what date of
service do you use? The date the consult comes in your door or the date
on the paperwork? Thanks!
Richard
Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
(860) 545-0174 Fax
Confidentiality Notice
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------------------------------
Message: 7
Date: Fri, 19 Jan 2007 14:39:06 -0500
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] Date of Service
To: "Richard Cartun" <Rcartun <@t> harthosp.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1CD6831EB9B26D45B0A3EAA79F7EBD32037D308E <@t> sjhaexc02.sjha.org>
Content-Type: text/plain; charset="utf-8"
the date we receive it
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Richard
Cartun
Sent: Friday, January 19, 2007 2:37 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Date of Service
When you receive a consult from another institution, what date of
service do you use? The date the consult comes in your door or the date
on the paperwork? Thanks!
Richard
Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
(860) 545-0174 Fax
Confidentiality Notice
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destroy all copies of the original message.
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------------------------------
Message: 8
Date: Fri, 19 Jan 2007 11:43:31 -0800
From: "Melissa Gonzalez" <Melissa.Gonzalez <@t> cellgenesys.com>
Subject: [Histonet] mouse DC marker on FFPE?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<A6B0506C2B000E45A7556B9A93C455477FF307 <@t> hqsvr01mail.cgi.com>
Content-Type: text/plain; charset="us-ascii"
Hi all,
I was wondering if anyone knows of a good product for dendritic cell
detection for mouse on formalin fixed paraffin embedded tissues? So far
I've had no luck with CD11c's (on paraffin sections). I was thinking of
trying one the human CD1a's (preferably not a mouse monoclonal) & seeing
if it cross-reacted. For example, we routinely use a rabbit x human CD3e
on mouse FFPE and it works fantastic.
Thanks a lot,
Melissa
------------------------------
Message: 9
Date: Fri, 19 Jan 2007 14:58:02 -0500
From: "Morken, Tim" <tim.morken <@t> thermofisher.com>
Subject: [Histonet] QC/QA IHC position open at ThermoFisher Scientific
- Lab Vision, California
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<AF18F2B78D0BE84788A9938D9817383A534F9A <@t> PGHCR-EXMB-VS-3.na.fshrnet.com>
Content-Type: text/plain; charset="us-ascii"
Here is a postion open at Lab Vision (a ThermoFisher Scientific
Company). It is all IHC with some sectioning involved. This is a small
group so everyone is involved in a variety of interesting projects
besides the QC work. Considering the cost of living in the San Francisco
area we will probably hire from the local (california) area. Please send
resumes to me at the address or email below.
QC Research Associate II
Summary of Job:
Performs quality control tests on antibodies, detection systems, and
ancillary products used for immunohistochemistry. Performs IHC and
works with Technical Support to test retainers and returned lots of
possible nonconforming products. Performs sectioning of paraffin tissue
blocks and frozen tissue blocks. May participate in special projects
involving development of new products, either reagent or
instrumentation.
Major Responsibilities:
Performs routine IHC testing of raw materials and manufactured antibody
products according to established quality control procedures/permanent
work instructions. Performs product IHC stability tests. Releases
product as directed by QC IHC Lab Manager. Performs experiments to
assess product or procedural problems under direction of QC IHC Lab
Manager and/or Technical Support Representative. Maintains and
manufactures positive control slide product inventory. Prepares
ancillary reagent/buffer products for IHC lab. Performs sectioning of
paraffin tissue blocks and frozen tissue blocks. Assists in maintenance
of tissue control stock. May be involved in special projects including
new product development, product optimization, and optimization
requested by sales rep/customers, either reagent or instrumentation.
Prepares reports on assigned assays, processes, or quality control
tests. Complies with GMP, QSRs, and ISO regulations. Assists in
maintaining laboratory equipment to assure accuracy and confidence in
performance, and reports equipment problems to Calibration
Representative. Complies with Lab Vision/NeoMarkers' Quality Policies
and Quality Procedures. Able to work closely with other departments in
reaching company goals.
Education or Equivalence of Experience:
BA/BS in Chemistry, Biochemistry, Biology, or Bioscience with 3 or more
years relevant experience. Able to perform all specific laboratory
procedures as requested by the QC IHC Lab Manager. Ability to meet
deadlines and ensure successful product release in a timely manner.
Must be able to write clear and understandable documentation. Good word
processing and spreadsheet skills. Familiar with antibody technology,
especially a good understanding of proteins, enzymes, and the structures
and functions of antibodies. Able to work in a team environment.
Certification as Histotechnologist (HTL) and Qualification for
Immunohistochemistry (QIHC) by American Society for Clinical Pathology
(ASCP) is desirable.
Supervisory Responsibilities: May oversee work of QC Research
Associate I.
Tim Morken
Technical Support Manager
Lab Vision - Neomarkers
ThermoFisher Scientific
47777 Warm Springs Blvd.
Fremont, CA 94539 USA
tel: 01-510-991-2840
fax: 01 510-991-2826
Tim.Morken <@t> thermofisher.com
web: www.labvision.com
www.thermofisher.com
------------------------------
Message: 10
Date: Fri, 19 Jan 2007 15:22:19 -0600
From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
Subject: [Histonet] Software needed for Shurmark cassette/slide
printer
To: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Cc: andrew.lisowski <@t> abbott.com
Message-ID:
<OF0288419C.AEC606DE-ON86257268.00752649-86257268.007570CF <@t> abbott.com>
Content-Type: text/plain; charset="US-ASCII"
A friend of mine (sort of) inherited a Shurmark Cassette and Slide
printer
- however, he was not able to get the software to run the equipment.
Does anyone have a copy of the software he can use to install in a PC?
Is
that possible?
Thanks -
Jackie O'
------------------------------
Message: 11
Date: Fri, 19 Jan 2007 14:27:21 -0800
From: "Tarango, Mark" <mtarango <@t> nvcancer.org>
Subject: RE: [Histonet] Date of Service
To: "Richard Cartun" <Rcartun <@t> harthosp.org>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<5AEC610C1CE02945BD63A395BA763EDE1F9926 <@t> NVCIEXCH02.NVCI.org>
Content-Type: text/plain; charset=us-ascii
Date it comes to us at our lab.
Mark Adam Tarango HT(ASCP)
Histology/Immunohistochemistry Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV 89135
mtarango <@t> nvcancer.org
Direct Line (702) 822-5112
Fax (702) 939-7663
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Friday, January 19, 2007 11:37 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Date of Service
When you receive a consult from another institution, what date of
service do you use? The date the consult comes in your door or the date
on the paperwork? Thanks!
Richard
Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
(860) 545-0174 Fax
Confidentiality Notice
This e-mail message, including any attachments, is for the sole use of
the intended recipient(s) and may contain confidential or proprietary
information which is legally privileged. Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are not the intended
recipient, please promptly contact the sender by reply e-mail and
destroy all copies of the original message.
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
"EMF <nvcancer.org>" made the following annotations.
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------------------------------
Message: 12
Date: Sat, 20 Jan 2007 08:10:53 -0800 (PST)
From: jayant limaye <jayant_limaye <@t> yahoo.com>
Subject: [Histonet] Collaboration Solutions for Clinical Labs and
BioPharma
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20070120161053.40358.qmail <@t> web51310.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello Histonet members,
Sending an email to this list as an alumni of UT
Southwestern and presently founder of a company called
Gencache which specializes in collaboration solutions
for Clinical labs and Biopharma.
Wish to personally connect with you, presenting you
with a proposal for your respective labs. **We also
wish to discuss opportunities of setting up a lab in
Orange County California, affiliated to your
respective labs.
We have recently helped develop a highly successful
end to end LIMS/LIS solution for an oncology lab in
Irvine, California.
We have been working with a lot of clinical
diagnostics and biotechnology companies in the West
coast(California) for the last 2 years. Started this
group after having been the Principal Software
Engineer R&D at Quest Diagnostics (Nasdaq: DGX).
We are presently based both in Irvine, California and
India.
We are planning on introducing a new Research Process
Management System. Please do let us know when you
would be interested in our platform. We would like to
have your teams as beta customers for our
Gencache RPMS (Research Process Management System)
which promises to be an exciting collaboration
platform for Research. We will customize the RPMS
for you and your affiliate organizations. This will
include custom Lab/Discovery Informatics as well.
You will also be very interested in our business
model.
We have a very unique Business model and proposition
to you (in fact please do look at our clientele of
diagnostic labs):
Only 1 Virtual Resource will be billed to your
individual lab at X dollars
per hour (this includes Process Management a key
factor in the above process, a Project Manager and a
multiplex of off-shore programmers. your lab billed
for only 1 virtual resource, no matter
number of extra developers working on the project).
Our primary revenue comes from consulting for
diagnostic labs, some of them prominently in the
cancer diagnostics space.
Here's an interview, the reason for sharing this
interview is to introduce my background, my work for
Quest Diagnostics and the Human Genome project amongst
others in the past.
http://www.socaltech.com/fullstory/0001300.html
Our team has grown and so have our product offerings
and services.
We hope we can share a fruitful and effective working
relationship with you in the near future.
Look forward to hearing back from you, Do call me at
(949)-273-0405.
Best Regards,
Jayant Limaye
Gencache LLC, Irvine, California
http://www.gencache.com
Collaboration Solutions for BioPharma
Phone: (949)-273-0405
------------------------------
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