[Histonet] Blue Nevus Diease-Help?
Scheherazade Humphrey
schraz13 <@t> yahoo.com
Thu Jan 18 14:29:13 CST 2007
Sorry I forgot to change the subject line for my message. Can anyone help me find a IHC stain the is performed on the disease Blue Nevus. I am having a hard time finding out if Fontana Masson is the correct one to use and also a possible case study to reference this
disease to. I had to reasearch this disease for a school project. If anyone could help me, your participation would be greatly appreciated. Thank You.
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Today's Topics:
1. RE:Information on Antibodies (Mark Elliott)
2. CK 19 (Dana Settembre)
3. Re: Help with a Reichert Jung Frigocut 2800
(Don.Birgerson <@t> leica-microsystems.com)
4. RE: Microwave procedure for monitoring temperature rep
roducibility (hymclab)
5. Re: Microwave procedure for monitoring temperature
reproducibility (Phil McArdle)
6. RE: Microwave procedure for monitoring temperature rep
roducibility (Inman, Anna)
7. RE: Microwave procedure for monitoringtemperature
reproducibility (Morken, Tim)
8. Re: Histonet Digest, Vol 38, Issue 26 (Scheherazade Humphrey)
9. RE: Re: Histonet Digest, Vol 38, Issue 26 (Terri Braud)
10. RE: Microwave procedure for monitoringtemperature
reproducibility (Chaussey, Leslie)
----------------------------------------------------------------------
Message: 1
Date: Thu, 18 Jan 2007 06:21:32 -0800
From: "Mark Elliott"
Subject: [Histonet] RE:Information on Antibodies
To: ,
Message-ID: <45AF11EC020000D6000306CE <@t> mail.mrl.ubc.ca>
Content-Type: text/plain; charset=US-ASCII
Marsha
I have used D2-40. What sort of info are you looking for?
MArk
>>> "Mildred Fail" 1/17/2007 10:01:10 AM >>>
Marsha
We use
2. Mesothelial clone HBME-1 from DAKO IVD AB
3. CK 19 clone b170 fromVector RUO Ab
Rena Fail
>>> "mprice26 <@t> juno.com" 01/17/07 12:15PM >>>
Hello in Histoland,
I need information on the following antibodies:
1. D2-40
2. HBME-1
3. CK-19
Thank you in advance.
Marsha Price, HT, QIHC (ASCP)
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------------------------------
Message: 2
Date: Thu, 18 Jan 2007 09:36:28 -0500
From: "Dana Settembre"
Subject: [Histonet] CK 19
To: , , "Mark
Elliott"
Message-ID:
Content-Type: text/plain; charset=US-ASCII
Hello Marsha,
I use CK 19 on FFPE human tissue.
I use Dako's cat.# M0888
It is an In Vitro Diagnostic antibody.
I use it at a dilution of 1:25 and incubate 15 mnutes RT
I also use their Target Retreival Solution
and use a papillary carcinoma as a positive control.
I get nice results.
Good Luck
Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ USA
>>> Mark Elliott 01/18/07 9:21 AM >>>
Marsha
I have used D2-40. What sort of info are you looking for?
MArk
>>> "Mildred Fail" 1/17/2007 10:01:10 AM >>>
Marsha
We use
2. Mesothelial clone HBME-1 from DAKO IVD AB
3. CK 19 clone b170 fromVector RUO Ab
Rena Fail
>>> "mprice26 <@t> juno.com" 01/17/07 12:15PM >>>
Hello in Histoland,
I need information on the following antibodies:
1. D2-40
2. HBME-1
3. CK-19
Thank you in advance.
Marsha Price, HT, QIHC (ASCP)
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------------------------------
Message: 3
Date: Thu, 18 Jan 2007 08:44:44 -0600
From: Don.Birgerson <@t> leica-microsystems.com
Subject: Re: [Histonet] Help with a Reichert Jung Frigocut 2800
To: Kurt Musselmann
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset=US-ASCII
Hello Kurt,
I believe we still have anti roll plates for most of our cryostats. I
will call you on the phone so we can play a game of twenty questions to
identify your holder.
Best regards,
Don
Don Birgerson
Leica Microsystems
Technical Assistance Center
Don.Birgerson <@t> Leica-Microsystems.Com
1-800-248-0123 ext 5918 (7:00 to 4:00 CT)
Kurt Musselmann
.nih.gov> To
Sent by:
histonet-bounces@ cc
lists.utsouthwest
ern.edu Subject
[Histonet] Help with a Reichart
Jung Frigocut 2800
01/18/2007 08:06
AM
Hello everybody
I am new to the list, and was directed to you guys by Alan Bright.
I have started using a Reichart Jung Frigocut 2800, and we have a badly
chipped anti-roll plate. Our plate measures roughly 5x3x0.3 cm, and I am
trying to find a replacement for it.
Does anybody know of a company that manufactures anti-roll plates of that
size, or would I have to change the whole anti-roll device (I had ordered
one from Electron Microscope Sciences (EMS) but it did not fit our machine
and had to be returned.
I appreciate any input
Kurt Musselmann
--
Kurt Musselmann Ph.D.
NIDCR/NIH
30 Convent Drive
Building 30, Room 403
Bethesda, MD 20892-4370
(301) 496 3065
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Message: 4
Date: Thu, 18 Jan 2007 08:51:49 -0600
From: hymclab
Subject: RE: [Histonet] Microwave procedure for monitoring temperature
rep roducibility
To: 'Zummak Melissa' ,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain
There is a CLSI (formerly NCCLS) Standard and Guideline book available:
Microwave Device Use in the Histology Laboratory; Approved Guidelines.
(description: This document provides recommendations for reproducing the
performance of microwave-accelereated procedures to prepare biological
specimens in the histology laboratory).
Go to the CLSI website for info: www.clsi.org.
I found this very helpful in fulfilling the new CAP requirements.
Dawn D. Schneider, HT(ASCP)
Howard Young Medical Center
Woodruff, WI 54568
(715)356-8174
schneiderd <@t> hyhc.com
-----Original Message-----
From: Zummak Melissa [mailto:Melissa.Zummak <@t> ssfhs.org]
Sent: Wednesday, January 17, 2007 10:34 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Microwave procedure for monitoring temperature
reproducibility
Hi everyone,
Does anyone know where I can find recommendations for checking\monitoring
temperature reproducibility for microwaves, I only use the microwave for
certain special stains currently.
Melissa Zummak
Alverno Clinincal Lab
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation.
------------------------------
Message: 5
Date: Thu, 18 Jan 2007 10:24:00 -0500
From: Phil McArdle
Subject: Re: [Histonet] Microwave procedure for monitoring temperature
reproducibility
To: Zummak Melissa
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <45AF9110.8080104 <@t> ebsciences.com>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Hello everyone:
And now a vendor weighs in:
We have several calibration and "accreditation-related" articles
available on our website (www.ebsciences.com ,
http://www.ebsciences.com/papers/microwave_quality.htm ,
http://www.ebsciences.com/pdf/EBS_CAP_RECOMMEND.pdf ).
In addition, we're happy to see that microwave use in the histology
laboratory is finally being given the scrutiny it deserves. Many of the
suggestions in CLSI's GP-28 guidelines (referenced in other postings)
are excellent, for example, troubleshooting, container placement, power
output determination, and overall, standardization of procedures and
elimination of variables. However, I feel there are some significant
problems with this publication; I have previously informed CLSI of my
primary concern:
Page 11, section 4.7: "A qualified authority CERTIFIED IN USING
MICROWAVE LEAKAGE INSTRUMENTATION [my emphasis] should determine
microwave leakage..." The problem is, to our knowledge, NO SUCH
CERTIFICATION EXISTS! Furthermore, despite repeated requests, CLSI has
not been forthcoming with suggestions. This is not to imply the absence
of extant legislation, Federal and otherwise, regulating the manufacture
and use of microwave devices; manufacturers have to comply with FDA and
other regulations regarding microwaves intended for in-vitro diagnostic
use. But as for leakage detection in the field, we have yet to come
across any relevant (or irrelevant, for that matter) Federal or State
certification. As you can imagine, for institutions deciding to adopt
the more stringent CLSI guidelines, this poses something of a "Catch-22."
In addition, page 17 outlines the use of a neon bulb array, for those
who aspire to eliminate "hot spots" in the microwave chamber. However,
while this can be a "fun" way to indirectly observe microwave energy,
unless the microwave were horribly defective, it is a fairly useless
exercise. Any object (including the array itself) placed in the
microwave chamber affects the distribution of microwaves, a classic
example of attempted observation affecting the phenomenon observed.
I want to stress, however, that overall, publication GP-28A is an
outstanding effort to provide "recommendations for quality assurance and
safety procedures for microwave equipment use" in their words, and to
help troubleshoot and eliminate variability in results of microwave usage.
Best regards,
Phil McArdle
--
Microwave Product Manager
Energy Beam Sciences, Inc.
29-B Kripes Rd.
East Granby, CT 06026
Tel: 800.992.9037 x 341
Fax: 860.653.0422
pmcardle <@t> ebsciences.com
www.ebsciences.com
You must be the change you want to see in the world.
- Mahatma Gandhi
NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it; please do the right thing and make it go away. Thank you.
Zummak Melissa wrote:
> Hi everyone,
>
> Does anyone know where I can find recommendations for
> checking\monitoring temperature reproducibility for microwaves, I only
> use the microwave for certain special stains currently.
>
> Melissa Zummak
> Alverno Clinincal Lab
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
------------------------------
Message: 6
Date: Thu, 18 Jan 2007 08:50:47 -0700
From: "Inman, Anna"
Subject: RE: [Histonet] Microwave procedure for monitoring temperature
rep roducibility
To: "hymclab" , "Zummak Melissa"
,
Message-ID:
<2925AE271EAAD440AF48FCCEB8002D090372F4C3 <@t> smgmail01.smgj.sclhs.net>
Content-Type: text/plain; charset="us-ascii"
What I recall about that publication......it doesn't really address
microwave usage for anything other than a specimen processing angle;
whereas, labs like mine only use it for heating agar/histogel to make
cell blocks and things........
Anna Inman, HT(ASCP)
Clinical Resource Specialist
SMH Pathology
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of hymclab
Sent: Thursday, January 18, 2007 7:52 AM
To: 'Zummak Melissa'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Microwave procedure for monitoring temperature
rep roducibility
There is a CLSI (formerly NCCLS) Standard and Guideline book available:
Microwave Device Use in the Histology Laboratory; Approved Guidelines.
(description: This document provides recommendations for reproducing the
performance of microwave-accelereated procedures to prepare biological
specimens in the histology laboratory).
Go to the CLSI website for info: www.clsi.org.
I found this very helpful in fulfilling the new CAP requirements.
Dawn D. Schneider, HT(ASCP)
Howard Young Medical Center
Woodruff, WI 54568
(715)356-8174
schneiderd <@t> hyhc.com
-----Original Message-----
From: Zummak Melissa [mailto:Melissa.Zummak <@t> ssfhs.org]
Sent: Wednesday, January 17, 2007 10:34 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Microwave procedure for monitoring temperature
reproducibility
Hi everyone,
Does anyone know where I can find recommendations for
checking\monitoring
temperature reproducibility for microwaves, I only use the microwave for
certain special stains currently.
Melissa Zummak
Alverno Clinincal Lab
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
CONFIDENTIALITY NOTICE: This e-mail communication and any attachments
may contain confidential and privileged information for the use of the
designated recipient(s) named above. If you are not the intended
recipient, you are hereby notified that you have received this
communication in error and that any review, disclosure, dissemination,
distribution or copying of it or its contents is prohibited. If you have
received this communication in error, please notify the sender at the
electronic mail address noted above and destroy all copies of this
communication and any attachments. Thank you for your cooperation.
_______________________________________________
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------------------------------
Message: 7
Date: Thu, 18 Jan 2007 11:33:53 -0500
From: "Morken, Tim"
Subject: RE: [Histonet] Microwave procedure for monitoringtemperature
reproducibility
To:
Message-ID:
Content-Type: text/plain; charset="us-ascii"
Phil wrote: "Page 11, section 4.7: "A qualified authority CERTIFIED IN
USING MICROWAVE LEAKAGE INSTRUMENTATION [my emphasis] should determine
microwave leakage..." The problem is, to our knowledge, NO SUCH
CERTIFICATION EXISTS! Furthermore, despite repeated requests, CLSI has
not been forthcoming with suggestions."
That's funny, but brings up an interesting question: Who does oversight
on a group like CLSI? If they went to the trouble of researching this
guideline, how is it that they never (apparently) realized there is no
such certification. What made them write such a requirement without
knowing if such a certification exisits? Did they just assume it
existed? And, if they did any lab work with an actual microwave, how
could they have done so without first having it checked by a person
certified to do so?
The final question. If CLSI can't figure that out, should we believe
them on anything else? This is a private group that has set itself up
as the final word on laboratory standardization. They charge massive
amounts of money for rather average informational documents (most of
which, these days, anyone can find on the internet for free with a
little persistance) and then something like this makes them look like
fools. It seems the only possible oversight is to refuse to buy
defective documents.
Actually, it kinda reminds me of another body that dreams up fantasy
questions to torture lab managers with...
Tim Morken
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Phil
McArdle
Sent: Thursday, January 18, 2007 7:24 AM
To: Zummak Melissa
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Microwave procedure for monitoringtemperature
reproducibility
Hello everyone:
And now a vendor weighs in:
We have several calibration and "accreditation-related" articles
available on our website (www.ebsciences.com ,
http://www.ebsciences.com/papers/microwave_quality.htm ,
http://www.ebsciences.com/pdf/EBS_CAP_RECOMMEND.pdf ).
In addition, we're happy to see that microwave use in the histology
laboratory is finally being given the scrutiny it deserves. Many of the
suggestions in CLSI's GP-28 guidelines (referenced in other postings)
are excellent, for example, troubleshooting, container placement, power
output determination, and overall, standardization of procedures and
elimination of variables. However, I feel there are some significant
=== message truncated ===
Scheherazade H.
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