[Histonet] Ventana IHC Staining

[Jesus Ellin]

To me it sounds that you need to get your mixers checked and make sure that they are not clogged. 


Jesus A. Ellin HT ASCP
Yuma Regional Medical Center
Histology Systems Technologist
Pathology Information Systems
928-336-7444 or 928-336-1144
Fax: 928-336-7319
 
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Janice Mahoney
Sent: Thursday, January 18, 2007 12:12 PM
To: histonet <@t> pathology.swmed.edu; Rene J Buesa
Subject: Re: [Histonet] Ventana IHC Staining

Oh Boy, here we go again.
I'm a Ventana user and when we have had this occasional problem it has been something simple like needing to do the routine maintenance.  Have you performed your function tests, checked your heat pads and decontaminated your instrument?  
I also have found the Ventana customer service people helpful.
Hope this helps you solve your problem.
Jan
Omaha

>>> Rene J Buesa <rjbuesa <@t> yahoo.com> 01/18/2007 12:46 PM >>>
Leslie:
  I suggest you to go to Histonet Archieves and find out about this very same recurrent problem with the Ventana Benchmark (and a very large and controversial thread about the likes and dislikes about Ventana).
  I personally do not like closed systems for IHC, I always used and preferred Dako and cannot help you on this problem. Check the Archieves!
  René J.

"Chaussey, Leslie" <Lchausse <@t> nmh.org> wrote:
  We're noticing some issues with our IHC staining. It's very random but we've been seeing instances where the positive control tissue stains fine but the patient tissue doesn't stain at all. In one instance, we re-ran the stain manually & it worked fine. We generally place patient tissue on the same slide as the control tissue using the red box control slides (red box is painted on the back of the slide). We have some ideas but wanted to check in to see if anyone else using Ventana Instrumentation (Benchmark XT) has seen that problem as well. Thanks for any feedback - it's greatly appreciated. 



Leslie J Chaussey






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