[Histonet] Re: Histonet Digest, Vol 38, Issue 26

Thu Jan 18 11:25:54 CST 2007

This is a link to a page containing some of the recent publications on blue nevus and its varying forms.  
http://www.thedoctorsdoctor.com/diseases/bluenevus.html#ipox
Since the diagnosis can be malignant to benign, with varying forms in between, microscopic evalutaion with stain correlation is key.  Also, because of the various forms, there are no single definitive stains.
Good luck with your project.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supv.
Laboratory, Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
(215) 938-3689

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
Scheherazade Humphrey
Sent: Thursday, January 18, 2007 9:56 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Histonet Digest, Vol 38, Issue 26

Can anyone help me find a IHC stain the is performed on the disease Blue Nevus. I am having a hard time finding out if Fontana Masson is the correct one to use and also a possible case study to reference this disease to. I had to reasearch this disease for a school project. If anyone could help me, your participation would be greatly appreciated. Thank You. 

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Today's Topics:

1. RE:Information on Antibodies (Mildred Fail)
2. EMPLOYMENT OPPROTUNITIES (Jessica Hirsch)
3. Luxol Reference (Tim Wheelock)
4. RE: CD133 Help!! (Tarango, Mark)
5. RE: CD133 Help!! (Tarango, Mark)
6. RE: CD133 Help!! (Makhijani, Nalini S)
7. LINES IN PARAFFIN SECTIONS (Tim Wheelock)
8. Re: LINES IN PARAFFIN SECTIONS (Rene J Buesa)
9. RE: LINES IN PARAFFIN SECTIONS (Weems, Joyce)
10. RE: LINES IN PARAFFIN SECTIONS (Rittman, Barry R)
11. Racemace/p63 cocktail (Dana Settembre)
12. looking for Andra Erickson (Patsy Ruegg)
13. Fwd: Re: [Histonet] LINES IN PARAFFIN SECTIONS (Gayle Callis)
14. LINES IN PARAFFIN SECTIONS 2 (Tim Wheelock)
15. RE: One more thing regarding Luxol Fast Blue differentiator
(Dennis Najarian)
16. FW: Re: [Histonet] Zeiss Microm Cryostat (D6900)
(Sherwood, Margaret )
17. Re: LINES IN PARAFFIN SECTIONS
(Jan.Minshew <@t> leica-microsystems.com)
18. Re: Re: [Histonet] Zeiss Microm Cryostat (D6900)
(Robert Chiovetti)
19. Help with a Reichart Jung Frigocut 2800 (Kurt Musselmann)
20. RE: Re: [Histonet] Zeiss Microm Cryostat (D6900) (Zummak Melissa)

----------------------------------------------------------------------

Message: 1
Date: Wed, 17 Jan 2007 13:01:10 -0500
From: "Mildred Fail" 
Subject: [Histonet] RE:Information on Antibodies
To: , 
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

Marsha
We use

2. Mesothelial clone HBME-1 from DAKO IVD AB 
3. CK 19 clone b170 fromVector RUO Ab

Rena Fail

>>> "mprice26 <@t> juno.com" 01/17/07 12:15PM >>>
Hello in Histoland,
I need information on the following antibodies:

1. D2-40

2. HBME-1

3. CK-19

Thank you in advance.

Marsha Price, HT, QIHC (ASCP)

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------------------------------

Message: 2
Date: Wed, 17 Jan 2007 13:20:35 -0500
From: "Jessica Hirsch" 
Subject: [Histonet] EMPLOYMENT OPPROTUNITIES 
To: 
Message-ID:

Content-Type: text/plain; charset="us-ascii"

CURRENT EMPLOYMENT OPPROTUNITIES

( HISTOLOGY, IMMUNOHISTOCHEMISTRY & PATHOLOGY )

I. Clarksburg, Maryland (Large Nationwide Clinical Labratory (division
of BioReference))

1. Histology Supervisor: 

Hours: Monday - Friday 12 midnight - 8:30am

Pay: based on experience, very competitive

Benefits: Medical, Dental, 401k

2. Histology Technologist

Hours: Monday - Friday 12 midnight - 8:30am 

Pay: based on experience, very competitive

Benefits: Medical, Dental, 401k

3. Grossers

Hours: Monday - Friday 7pm-11:30pm 

Pay: ~$30/hr

II. Elmwood Park, New Jersey ( Nationwide Clinical Laboratory)

1. Histology Technologist ( 2 positions available, immediate start )

Hours: Monday - Friday, 9:00pm - 5:30am 

Pay: Negotiable

Benefits: Medical, Dental, 401k

2. ImmunoHistoChemistry Supervisor 

Hours: Monday - Friday, 7am or 8am - 4pm or 5pm 

Pay: Negotiable

Benefits: Medical, Dental, 401k

3. Generalist

Hours: Monday - Friday, Day Shift

Pay: Negotiable

Benefits: Medical, Dental, 401k

III. New York City, New York ( large world renowned Cancer Hospital )

1. Pathologist Assistant

Hours: Monday - Friday, Day shift, Full-Time

Pay: $60k - $80k based on experience

Benefits: Major Medical, Dental, Vision, 401k, etc.

2. Histology Technologist

Hours: Monday - Friday (shift to be announced) 

Pay: negotiable

Benefits: Medical, Dental, 401k

For immediate consideration for any position:

Send resumes to Jessica <@t> medstaffservices.com
OR fax to 732.238.2152

For questions call: 

Jessica Hirsch at 732.238.6050 ext.58

Jessica D. Hirsch
Staffing Specialist
Medical Staffing Services, Inc.
557 Cranbury Road
East Brunswick, NJ 08816
P: (732) 238-6050 x58
F: (732) 238-2152
Jessica <@t> medstaffservices.com 
http://www.medstaffservices.com 

------------------------------

Message: 3
Date: Wed, 17 Jan 2007 13:50:20 -0500
From: Tim Wheelock 
Subject: [Histonet] Luxol Reference
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <45AE6FEC.3000703 <@t> mclean.harvard.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Hi Gayle:

As I was mentioning to Andi, I myself cannot find a reference for the 
protocol. 
I was taught the stain at the Massachusetts General Hospital back in 1984. 
(It was simply typed on a 3 x 5 file card) 
I took a copy of the recipe with me when I left the General in 1986.
The neuropathology lab was liquidated some years ago, and it's functions 
integrated into general pathology.
Perhaps, someone in Surgical Pathology or Special Stains at MGH could 
tract it down.
The main number at MGH is 617-726-2000

It is very interesting though that the exact same "differentiator" of 
the Luxol (1% hydroquinone, 5% sodium sulfite, aqueous) is used for the 
reducing agent in the Bodian protein-silver stain.
We used it for both purposes at the MGH., although I use the 
Bielschowsky silver stain instead here at McLean
One can find find a reference for the Bodian, at least, in Sheehan's 
"Theory and Practice of Histotechnology", second edition,, page 256.

By the way, In my first email I did not mean to disparage the use of 
Lithium Carbonate.
Every version of the stain I have seen in the Histology books uses Lithium.
So it must work perfectly well in most labs, such as your own.
I see my protocol as just an alternative to Lithium just as the Lithium 
would definitely be an alternative for me if I had problems with the 
Hydroquinone.

Thank you for the detailed description of the Lithium Carbonate 
technique; I will keep it on hand.

Tim

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------------------------------

Message: 4
Date: Wed, 17 Jan 2007 10:45:21 -0800
From: "Tarango, Mark" 
Subject: RE: [Histonet] CD133 Help!!
To: "Makhijani, Nalini S" ,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<5AEC610C1CE02945BD63A395BA763EDE1F990D <@t> NVCIEXCH02.NVCI.org>
Content-Type: text/plain; charset=us-ascii

I tried that, but no luck. Did you have some success incubating the
primary overnight?

Mark Adam Tarango HT(ASCP)

Histology/Immunohistochemistry Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV 89135

mtarango <@t> nvcancer.org

Direct Line (702) 822-5112

Fax (702) 939-7663

-----Original Message-----
From: Makhijani, Nalini S [mailto:Nalini.Makhijani <@t> va.gov] 
Sent: Wednesday, January 17, 2007 8:08 AM
To: Tarango, Mark; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] CD133 Help!!

Try letting it go overnight.
Nalini Makhijani

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tarango,
Mark
Sent: Tuesday, January 16, 2007 5:54 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD133 Help!!

If anyone has a protocol for CD133 on paraffin OR frozen tissue that
works, please send it to me! I've tried so many times w/ this antibody
and I'm having a hell of a time getting any staining.

Thanks!!

Mark Adam Tarango HT(ASCP)

Histology/Immunohistochemistry Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV 89135

mtarango <@t> nvcancer.org 

Direct Line (702) 822-5112

Fax (702) 939-7663

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Message: 5
Date: Wed, 17 Jan 2007 10:47:38 -0800
From: "Tarango, Mark" 
Subject: RE: [Histonet] CD133 Help!!
To: "Patsy Ruegg" 
, "Makhijani, Nalini S"
, histonet <@t> lists.utsouthwestern.edu
Message-ID:
<5AEC610C1CE02945BD63A395BA763EDE1F990E <@t> NVCIEXCH02.NVCI.org>
Content-Type: text/plain; charset=us-ascii

I have 3 different CD133/AC133 antibodies. I can't get even one of them
to work. I'll have to buy the one you suggest.

Thanks

Mark Adam Tarango HT(ASCP)

Histology/Immunohistochemistry Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV 89135

mtarango <@t> nvcancer.org

Direct Line (702) 822-5112

Fax (702) 939-7663

-----Original Message-----
From: Patsy Ruegg [mailto:pruegg <@t> ihctech.net] 
Sent: Wednesday, January 17, 2007 8:36 AM
To: 'Makhijani, Nalini S'; Tarango, Mark;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] CD133 Help!!

Which cd133 are you using? If you are using the antibody from Miltyeni
I
suggest you toss it and get the one from Abcam rab polyclonal ab19898, I
use
this at 1:100 overnight after HIER steam in HpH buffer for 25 min. with
rab
labeled polymer hrp detection for 45 min. and dab for 15 min.

This antibody as well as many others has been discussed on the NSH IHC
Resource Group list serve, to join this group go to www.ihcrg.org and
submit
a membership form online. We have a list serve similar to histonet that
focuses just on IHC/Molecular Diagnostics isssues.

Patsy Ruegg
Chair, IHCRG

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Makhijani,
Nalini S
Sent: Wednesday, January 17, 2007 9:08 AM
To: Tarango, Mark; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] CD133 Help!!

Try letting it go overnight.
Nalini Makhijani

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tarango,
Mark
Sent: Tuesday, January 16, 2007 5:54 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD133 Help!!

If anyone has a protocol for CD133 on paraffin OR frozen tissue that
works, please send it to me! I've tried so many times w/ this antibody
and I'm having a hell of a time getting any staining.

Thanks!!

Mark Adam Tarango HT(ASCP)

Histology/Immunohistochemistry Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV 89135

mtarango <@t> nvcancer.org 

Direct Line (702) 822-5112

Fax (702) 939-7663

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Message: 6
Date: Wed, 17 Jan 2007 11:48:46 -0800
From: "Makhijani, Nalini S" 
Subject: RE: [Histonet] CD133 Help!!
To: "Tarango, Mark" ,

Message-ID:
<715FA772CEA81A47B1A762AB6E45123A2733D7 <@t> VHAV22MSGA3.v22.med.va.gov>
Content-Type: text/plain; charset="us-ascii"

I've never used this antibody- I was just suggesting a general technique
that I have seen work with overnight incubation rather than with a 1
hour
incubation, e.g.with Pecam 1.

-----Original Message-----
From: Tarango, Mark [mailto:mtarango <@t> nvcancer.org] 
Sent: Wednesday, January 17, 2007 10:45 AM
To: Makhijani, Nalini S; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] CD133 Help!!

I tried that, but no luck. Did you have some success incubating the
primary overnight?

Mark Adam Tarango HT(ASCP)

Histology/Immunohistochemistry Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV 89135

mtarango <@t> nvcancer.org

Direct Line (702) 822-5112

Fax (702) 939-7663

-----Original Message-----
From: Makhijani, Nalini S [mailto:Nalini.Makhijani <@t> va.gov] 
Sent: Wednesday, January 17, 2007 8:08 AM
To: Tarango, Mark; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] CD133 Help!!

Try letting it go overnight.
Nalini Makhijani

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tarango,
Mark
Sent: Tuesday, January 16, 2007 5:54 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD133 Help!!

If anyone has a protocol for CD133 on paraffin OR frozen tissue that
works, please send it to me! I've tried so many times w/ this antibody
and I'm having a hell of a time getting any staining.

Thanks!!

Mark Adam Tarango HT(ASCP)

Histology/Immunohistochemistry Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV 89135

mtarango <@t> nvcancer.org 

Direct Line (702) 822-5112

Fax (702) 939-7663

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Message: 7
Date: Wed, 17 Jan 2007 16:02:20 -0500
From: Tim Wheelock 
Subject: [Histonet] LINES IN PARAFFIN SECTIONS
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <45AE8EDC.7020903 <@t> mclean.harvard.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Hi Everyone:

I have a serious problem with lines/splits forming in my 5 micron 
sections as they come off the microtome.
I just started using Paraplast Xtra Tissue Embedding Medium.
My infiltrating wax is regular Paraplast.
My disposable blades are Surgipath high profile Teflon coated blades.
The microtome is a Microm HM 355S.
I cut human brain tissue exclusively.

I had been using Surgipath embedding media.
I decided to try the X-tra because I had been having problems with the 
sections being thick and thin on just one side of the section.
Also the wax seeming to be thicker (more opaque) on one side of the block.
The blocks of tissue also were not laying flat in the mold, hence a lot 
of trimming after.
There was some problem with lines even with the Surgipath but it was 
intermittent., and not nearly as bad as the X-tra.

I notice that trimming requires more physical effort with the Paraplast 
X-tra.
And I have had much more of a problem with lines than with the Surgipath 
Embedding Media.
Does it have a higher % of plastic in it.

I cleaned out the reservoir on the embedding center and played with the 
knife angle but to no avail.
I am not sure if the lines have to do with the wax or the blades or both.

Thank you for any advice you can give me.

Tim Wheelock
Harvard Brain Tissue Resource Center
McLean Hospital

I

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Scheherazade H. 

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