Fwd: Re: [Histonet] LINES IN PARAFFIN SECTIONS

[Gayle Callis]

>Stir the paraffin just before embedding to redistribute the plastic 
>polymers and other additives in paraffin.  These tend to settle out at 
>melting and being heavier, will settle to bottom of the dispenser.  Little 
>blobs of stuff often appear, but more likely end up in your embedding 
>molds, creating problems.   A cheap spatula does the trick.


We just encountered this problem with another brand of paraffin - stirring 
cured the problem.


Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)