[Histonet] LINES IN PARAFFIN SECTIONS
twheelock <@t> mclean.harvard.edu
Wed Jan 17 15:02:20 CST 2007
I have a serious problem with lines/splits forming in my 5 micron
sections as they come off the microtome.
I just started using Paraplast Xtra Tissue Embedding Medium.
My infiltrating wax is regular Paraplast.
My disposable blades are Surgipath high profile Teflon coated blades.
The microtome is a Microm HM 355S.
I cut human brain tissue exclusively.
I had been using Surgipath embedding media.
I decided to try the X-tra because I had been having problems with the
sections being thick and thin on just one side of the section.
Also the wax seeming to be thicker (more opaque) on one side of the block.
The blocks of tissue also were not laying flat in the mold, hence a lot
of trimming after.
There was some problem with lines even with the Surgipath but it was
intermittent., and not nearly as bad as the X-tra.
I notice that trimming requires more physical effort with the Paraplast
And I have had much more of a problem with lines than with the Surgipath
Does it have a higher % of plastic in it.
I cleaned out the reservoir on the embedding center and played with the
knife angle but to no avail.
I am not sure if the lines have to do with the wax or the blades or both.
Thank you for any advice you can give me.
Harvard Brain Tissue Resource Center
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