[Histonet] Luxol Reference

[Tim Wheelock]

Hi Gayle:

As I was mentioning to Andi, I myself cannot find a reference for the 
protocol. 
I was taught the stain at the Massachusetts General Hospital back in 1984. 
(It was simply typed on a 3 x 5 file card) 
I took a copy of the recipe with me when I left the General in 1986.
The neuropathology lab was liquidated some years ago, and it's functions 
integrated into general pathology.
Perhaps, someone in Surgical Pathology or Special Stains at MGH could 
tract it down.
The main number at MGH is 617-726-2000

It is very interesting though that the exact same "differentiator" of 
the Luxol (1% hydroquinone, 5% sodium sulfite, aqueous) is used for the 
reducing agent in the Bodian protein-silver stain.
We used it for both purposes at the MGH., although I use the 
Bielschowsky silver stain instead here at McLean
One can find find a reference for the Bodian, at least, in Sheehan's 
"Theory and Practice of Histotechnology", second edition,, page 256.

By the way, In my first email I did not mean to disparage the use  of 
Lithium Carbonate.
Every version of the stain I have seen in the Histology books uses Lithium.
So it must work perfectly well in most labs, such as your own.
I see my protocol as just an alternative to Lithium just as the Lithium 
would definitely be an alternative for me if I had problems with the 
Hydroquinone.

 

Thank you for the detailed description of the Lithium Carbonate 
technique; I will keep it on hand.

Tim
 


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