[Histonet] VEGFR1 and 2 in rat

Patsy Ruegg pruegg <@t> ihctech.net
Wed Jan 17 10:53:15 CST 2007

I use Flt-1/Vegfr1 and Flk-1/KDR/Vegfr2 from Lab Vision now ThermoFisher cat
#RB1527 andRB9239 respectively.  For both, HIER 20' with citrate buffer ab
dilution 1:25 - 1:50 for 60' with rab labeled polymer hrp for 30' and dab
10' on rat tissue.  Angiosarcoma is a good control for these.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Boor Peter
Sent: Tuesday, January 16, 2007 11:01 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] VEGFR1 and 2 in rat

Can anyone recommend an antibody for VEGFR 1 and 2 that works well in rat?
We only tested anti-human polyclonals (R&D) with various Ag retrieval
protocols with no result. 
Also, there are Ab against human phosphorylated VEGFR that should be
apliable for IH (Calbiochem), do they work also in rat tissue?
Thanks a lot for any information!
Peter Boor

Peter Boor

University Clinic of Aachen, Dept. of Nephrology and Immunology, Pauwelsstr.
30;  52074 Aachen; Germany   

Home:   Kullenhofstr. 54A, App. 549; 52074 Aachen; Germany

Mob.: 0049 (0) 177 2345 163

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Fax: 0049 (0) 241 80 82446

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