[Histonet] CD133 Help!!

Rene J Buesa rjbuesa <@t> yahoo.com
Wed Jan 17 10:23:30 CST 2007

  I have not worked with this Ab but as a rule, when I did not get a clear reaction, I used to work with the HIER pH or the dilution, rather than with the incubation time. No matter how long you increase the reaction time, if there is not enough Ab concentration (or epitope signal for that matter) you will not get an intense result.
  René J.

"Tarango, Mark" <mtarango <@t> nvcancer.org> wrote:
  If anyone has a protocol for CD133 on paraffin OR frozen tissue that
works, please send it to me! I've tried so many times w/ this antibody
and I'm having a hell of a time getting any staining.


Mark Adam Tarango HT(ASCP)

Histology/Immunohistochemistry Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV 89135

mtarango <@t> nvcancer.org 

Direct Line (702) 822-5112

Fax (702) 939-7663

"EMF " made the following annotations.
CONFIDENTIALITY NOTICE: This e-mail message, including any
attachments, is for the sole use of the intended 
recipient(s) and may contain confidential, proprietary, 
and/or privileged information protected by law. If you are 
not the intended recipient, you may not use, copy, or 
distribute this e-mail message or its attachments. If you 
believe you have received this e-mail message in error, 
please contact the sender by reply e-mail and destroy all 
copies of the original message
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

Don't pick lemons.
See all the new 2007 cars at Yahoo! Autos.

More information about the Histonet mailing list