[Histonet] "Home made" IF transport media

[mtitford <@t> aol.com]

 Robert Fauck in New Zealand asks about "home brewed" immunofluorescence transport media.
 
Dr Elias gave a simlier formula in the very first issue of the Journal of Histotechnology ("New method for shipment of renal biopsies",  Elias. J.M., et al.  J. Histotechnol 1: 15-16 1977) His was a two part solution of a buffer, and a working solution:
 
Stock buffer
1M Potassium citrate buffer pH 7.0....2.5ml
0.1M Magnesium sulphate................5.0ml
0.1M N-ethyl maleimide....................5.0ml
Distilled water..............................87.5ml
(Adjust to pH 7.0 with 1M KOH)
 
Working solution
Ammonium sulphate (NH4)2SO4...................55 grams
Stock buffer..............................................100 ml
 
Hope this helps
 
Mike Titford
USA Pathology
Mobile AL USA
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