[Histonet] RE: Histonet Digest, Vol 38, Issue 22

[Pixley, Sarah (pixleysk)]

Dear Stephen Clark:

If by OE, you mean olfactory epithelium, we stain that frequently for
BrdU. My first question is, are you decalcifying, and with what? That
will critically affect the success of your staining. We have found that
you can use either EDTA or formic acid decalcification, but we could NOT
get BrdU staining after using other treatments, like the rapid (probably
sulfuric acid) decalcifiers. But if you had success with fluorescence,
you should have it with DAB. Email me privately and I will be happy to
send you some methods.

Sarah Pixley,
Sarah.pixley <@t> uc.edu


Message: 1
Date: Sun, 14 Jan 2007 13:37:04 -0600
From: "Stephen Clark" <sclark59 <@t> hotmail.com>
Subject: [Histonet] DAB brdU protocol
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY108-DAV119162175CCB83C3DB4EEAC7B60 <@t> phx.gbl>
Content-Type: text/plain;	charset="iso-8859-1"

Does anyone have a protocol for DAB staining, specifically for mouse OE
sections at about 10 or 18um using brdU?  I had been using fluorescent
staining procedures, but i wanted to try something non-flourescent to
elimate any problems with autoflourescence.  I have tried it a few times
already with a rough protocol supplied by a colleague with little
success.