[Histonet] paraffin embedded tissue for EM
suetrant <@t> telus.net
Mon Jan 15 21:17:06 CST 2007
I also have taken paraffin-B5 fixed embedded blocks back to a state that I
can use for electron microscopy. I have had very good results, but it is a
long process of approximately 8 hours.
You need to use 2-3 changes of xylenes, followed by graded alcohols starting
with 100 % and tapering to 25%. This is followed by distilled water. You
can then post fix in a 3.5% gluteraldehyde (fixed for 2 days), and proceed
through a regime of your regular fixation with an OsO4 solution, etc.
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