[Histonet] TEM for paraffin embedded tissue section??
Rene J Buesa
rjbuesa <@t> yahoo.com
Mon Jan 15 09:30:30 CST 2007
In extreme cases (then there was no more material in the block) we have done that.
Place the the embedding cone (with the tip removed) with the not cured plastic over the area of the section you want to process. Holding both secure together, cure the plastic as usual.
When curing is completed remove the cone that will now include the area of the section stuck to the cured resine.
With extreme care orient the cone and section it with the ultramicrotome. Stain as usual with your opaque stain.
Fixation will not be ideal, but some useful images can be obtained.
Hope this will help you.
Choi Ul Soo <cytologer <@t> msn.com> wrote:
Hello Dear Histonetters!
would anyone there tell me the story about using paraffin embedded tissue
for TEM analysis?
I have seen several reports reading that they used paraffin embedded
transmisison electron microscopy, but our technician is not familiar to
Would the results be consistent regardless of the objects of interest,
tissues? I'd like to get findings indicative of neuroendocrine origins from
lung mass fixed in formalin, and paraffin embedded tisse section.
Please share your experiences and give me advice.
Any inputs would be very much appreciated.
Ul Soo Choi
VMTH 208, CVM, Seoul National University, Shilim9 dong, Gwanakgu,
Seoul, Korea 151-742
¿À´Ã ¹«½¼ ÀÏÀÌ »ý±æ±î ±Ã±ÝÇÏ½ÃÁÒ? MSN ¿î¼¼¿¡¼­ È®ÀÎÇÏ¼¼¿ä.
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