[Histonet] TEM for paraffin embedded tissue section??

P Pierce contact <@t> excaliburpathology.com
Mon Jan 15 08:41:10 CST 2007

Paraffin sections will not survive the electron beam. 
You will need to melt out the paraffin and dissolve out the remaining paraffin in the tissue with xylene. 
After removing the paraffin, the tissue will need to be taken back through alcohols and rehydrated.
Then it will need to be post-fixed in osmium and processed through to a plastic embedding media such as epon or araldite.

The micrograph quality will not be as good as the tissue initially being fixed in gluteraldehyde, but it can be done.

Paula Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc. 
630 N. Broadway 
Moore, OK 73160 
contact <@t> excaliburpathology.com

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