[Histonet] Cryostats for insect neuroanatomy?

[Hao Li]

Hello,

I want to do silver staining in Drosophila and I have looked at a number of
published protocols. One thing I have noticed is that practically all of
them use 
paraffin or plastics sectioning. The problem is that we have only a cryostat
in 
the lab. So is there anything wrong with cryostats? For example, I have been
worrying that perhaps the 30% sucrose treatment will shrink and distort the 
tissue? Thanks for your help!

Hao Li
Institute of Neuroscience, CAS