[Histonet] RE: fluorescent mouse to mouse

[C.M. van der Loos]

   Dear Tara,

   A  Zenon  kit ([1]www.invitrogen.com and search for "zenon") is a good
   suggestion.   A  fluorescein  (or  Alexa)  conjugated  anti-mouse  Fab
   fragment  is  in  vitro  bound  to  your primary. After completing the
   "conjugation"  incubate  your  section  with  the antibody complex. Be
   aware  you  have  to  check  your mouse isotype before purchasing. The
   protocol  for  labeling  is not included with the kit we purchased (!)
   and appeared also very difficult to find at their website (ooops): use
   5 ul of labeling reagent per ug of mouse IgG (5 min, RT), block with 5
   ul  of blocking reagent (5 min, RT); then prepare dilution(s). I would
   not advise an overnight 4C incubation; just 30-60 min at RT.

   Lots of success!

   Chris van der Loos, PhD
   Dept. of Pathology
   Academic Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands


   Date: Thu, 11 Jan 2007 10:12:25 -0800 (PST)
   From: Tara Bullard <tbullard1231 <@t> yahoo.com>
   Subject: [Histonet] fluorescent mouse to mouse
   To: histonet <@t> lists.utsouthwestern.edu
   Hello All,
        I  am  new to the list.  I have 2 separate questions which I will
   spost separately.
       The  first  question-  is  there  a  good  kit  for mouse to mouse
   fluorescent immunohistochemistry?

     Thank you,
     Tara

References

   1. http://www.invitrogen.com/