[Histonet] RE: fluorescent mouse to mouse
C.M. van der Loos
c.m.vanderloos <@t> amc.uva.nl
Fri Jan 12 08:35:25 CST 2007
Dear Tara,
A Zenon kit ([1]www.invitrogen.com and search for "zenon") is a good
suggestion. A fluorescein (or Alexa) conjugated anti-mouse Fab
fragment is in vitro bound to your primary. After completing the
"conjugation" incubate your section with the antibody complex. Be
aware you have to check your mouse isotype before purchasing. The
protocol for labeling is not included with the kit we purchased (!)
and appeared also very difficult to find at their website (ooops): use
5 ul of labeling reagent per ug of mouse IgG (5 min, RT), block with 5
ul of blocking reagent (5 min, RT); then prepare dilution(s). I would
not advise an overnight 4C incubation; just 30-60 min at RT.
Lots of success!
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands
Date: Thu, 11 Jan 2007 10:12:25 -0800 (PST)
From: Tara Bullard <tbullard1231 <@t> yahoo.com>
Subject: [Histonet] fluorescent mouse to mouse
To: histonet <@t> lists.utsouthwestern.edu
Hello All,
I am new to the list. I have 2 separate questions which I will
spost separately.
The first question- is there a good kit for mouse to mouse
fluorescent immunohistochemistry?
Thank you,
Tara
References
1. http://www.invitrogen.com/
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