[Histonet] F4/80 in FFPE mouse tissues

[Horn, Diane A]

I am searching for some advice on IHC with F4/80 on mouse tissues.  The
antibody is from Serotec diluted 1:50 in 2% BSA/PBS.  The kidney tissues
have been fixed in 4% paraformaldehyde and the liver has been fixed in
10% NFB.  The liver stains fine but the kidney has high background and
no positive staining for F4/80.  I am using a kit from Biocare
(rat-on-mouse) that is a biotin free system.  The only other thing is
the kidney sections were cut about 1-2 months ago and stored in a slide
box in the -20 freezer (not frost free).  Any advice or protocol would
be appreciated!

 

Thanks to all,

Diane

 

Diane A. Horn

Department of Pathology

UTHSCSA

7703 Floyd Curl Dr.

San Antonio, TX  78229

210-567-4039