[Histonet] HOPE fixation
bhewlett <@t> cogeco.ca
Fri Jan 5 13:30:50 CST 2007
HOPE =Hepes-glutamic acid buffer mediated Organic solvent Protection Effect.
Tissues are placed in this non-fixative buffer at 4C O/N, then
fixed/dehydrated in acetone and transferred to paraffin.
Here is a website http://www.hope-fixation.com/
----- Original Message -----
From: "Kim Merriam" <kmerriam2003 <@t> yahoo.com>
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, January 05, 2007 2:13 PM
Subject: [Histonet] HOPE fixation
OK, I know someone on this list must know the answer to this. What is HOPE
fixation? I am reading a journal article that refers to this procedure. I
have done a Pubmed search, and I can find several references to it, but no
actual protocol on how to make the stuff up (maybe you need to buy it?).
I am not sure if I would ever be trying this method, but inquiring minds
want to know!
Kim Merriam, MA, HT(ASCP)
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