[Histonet] HOPE fixation

Bryan Hewlett bhewlett <@t> cogeco.ca
Fri Jan 5 13:30:50 CST 2007


HOPE =Hepes-glutamic acid buffer mediated Organic solvent Protection Effect.
Tissues are placed in this non-fixative buffer at 4C O/N, then 
fixed/dehydrated  in acetone and transferred to paraffin.

Here is a website http://www.hope-fixation.com/


----- Original Message ----- 
From: "Kim Merriam" <kmerriam2003 <@t> yahoo.com>
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, January 05, 2007 2:13 PM
Subject: [Histonet] HOPE fixation

OK, I know someone on this list must know the answer to this.  What is HOPE 
fixation?  I am reading a journal article that refers to this procedure.  I 
have done a Pubmed search, and I can find several references to it, but no 
actual protocol on how to make the stuff up (maybe you need to buy it?).

I am not sure if I would ever be trying this method, but inquiring minds 
want to know!


Kim Merriam, MA, HT(ASCP)
Cambridge, MA

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